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| Status |
Public on May 08, 2019 |
| Title |
KH-type splicing regulatory protein controls colorectal cancer cell growth and modulates the tumor microenvironment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
KH-type splicing regulatory protein (KHSRP) is a multifunctional nucleic acid binding protein. KHSRP regulates transcription, mRNA decay and translation, and miRNA biogenesis that influence distinct functions associated with cancer cell biology, such as inflammation and cell-fate determination. Our study uncovered novel mechanistic-based data on the tumor-promoting effects of KHSRP in colorectal cancer.
We used microarrays to detail changes in gene expression upon specific knockdown of KHSRP in SW480 cells
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| |
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| Overall design |
SW480 cells were transiently transfected with KHSRP siRNA for RNA extraction and hybridization on Affymetrix microarrays.
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| |
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| Contributor(s) |
Caiazza F, Ryan EJ |
| Citation(s) |
31404541 |
| Submission date |
Mar 26, 2018 |
| Last update date |
Aug 14, 2019 |
| Contact name |
Francesco Caiazza |
| E-mail(s) |
francesco.caiazza@gmail.com
|
| Organization name |
UCSF
|
| Department |
Pharmaceutical Chemistry
|
| Lab |
Charles Craik
|
| Street address |
600 16th street, Room S-514
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA445699 |
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