|
| Status |
Public on Oct 30, 2018 |
| Title |
siRNA knockdown of neonatal rat cardiac myocytes and fibroblasts |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Primary neonatal rat cardiac myocytes or fibroblasts were isolated and subjected to siRNA mediated Yap knockdown
|
| |
|
| Overall design |
Primary cardiac myocytes and fibroblasts were isolated from two-day old rats via neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol followed by percoll gradient separation. Cells were transfected with siRNAs designed against Yap, or universal negative control siRNAs. Transfected cells were collected 48 hours post transfection for RNAseq.
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| |
|
| Contributor(s) |
Flinn MA, O'meara CC, Link BA |
| Citation(s) |
30295714 |
| |
| Submission date |
Mar 28, 2018 |
| Last update date |
Oct 30, 2018 |
| Contact name |
Michael Andrew Flinn |
| Organization name |
Medical College of Wisconsin
|
| Department |
Physiology
|
| Lab |
O'Meara Lab
|
| Street address |
8701 W Watertown Plank Rd
|
| City |
Wauwatosa |
| State/province |
WI |
| ZIP/Postal code |
53226 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA446648 |
| SRA |
SRP136732 |
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