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| Status |
Public on Apr 07, 2018 |
| Title |
Identification of direct transcriptional target genes of ExoS/ChvI two-component signaling in Sinorhizobium meliloti |
| Platform organisms |
Sinorhizobium meliloti; Medicago truncatula |
| Sample organism |
Sinorhizobium meliloti |
| Experiment type |
Expression profiling by array
|
| Summary |
We performed transcriptional profiling with chvI gain-of-function (EC220) and reduced-function (EC69) strains. The chvI gain-of-function strain that we used contains a dominant gain-of-function chvI allele in addition to wild-type chvI. We identified genes that, relative to their expression level in the wild type, are both upregulated in the chvI gain-of-function strain and downregulated in the reduced-function strain or vice versa.
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| Overall design |
Gene expression profiling of Sinorhizobium meliloti Rm1021-derived strains, with wild type or mutant chvI alleles, using custom Affymetrix GeneChips
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| Contributor(s) |
Chen EJ, Long SR |
| Citation(s) |
19749054 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM093628 |
Control of Symbiotic Gene Expression in Sinorhizobium meliloti |
STANFORD UNIVERSITY |
SHARON R LONG |
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| |
| Submission date |
Apr 06, 2018 |
| Last update date |
Apr 09, 2018 |
| Contact name |
Melanie J Barnett |
| Organization name |
Stanford University
|
| Department |
Biology
|
| Lab |
Sharon R. Long
|
| Street address |
371 Jane Stanford Way
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL9757 |
[stSmel_Mtra] SRL_SU Sinorhizobium meliloti/Medicago truncatula dual-genome SymbiosisChip, Affymetrix part number 510699 |
|
| Samples (9)
|
| GSM3084286 |
Sinorhizobium meliloti EC69, biological rep1 |
| GSM3084287 |
Sinorhizobium meliloti EC69, biological rep2 |
| GSM3084288 |
Sinorhizobium meliloti EC69, biological rep3 |
| GSM3084289 |
Sinorhizobium meliloti EC176, biological rep1 |
| GSM3084290 |
Sinorhizobium meliloti EC176, biological rep2 |
| GSM3084291 |
Sinorhizobium meliloti EC176, biological rep3 |
| GSM3084292 |
Sinorhizobium meliloti EC220, biological rep1 |
| GSM3084293 |
Sinorhizobium meliloti EC220, biological rep2 |
| GSM3084294 |
Sinorhizobium meliloti EC220, biological rep3 |
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| Relations |
| BioProject |
PRJNA449085 |
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