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Series GSE113155 Query DataSets for GSE113155
Status Public on Apr 17, 2018
Title Transcriptome analysis by microarray of human placental chorionic villi explants infected with Trypanosoma cruzi
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Chagas’ disease, one of the major public health concerns in Latin America, is caused by the haemophlagelated protozoan Trypanosoma cruzi (T. cruzi). In the past few years congenital transmission of T. cruzi has become more important, and partly responsible for the “globalization of Chagas’ disease”. The congenital transmission, although with low rates, represents the main route of transmission in non-endemic countries and endemic countries without vectorial transmission, and represents one third of the new cases each year. Diverse pathogens, including T. cruzi, are able to cross the placental barrier and infect both the placenta and fetus. However, the exact cellular and molecular mechanisms of host-pathogen interaction between T. cruzi and the placenta has been scarcely studied. The use of microarray analysis to determine expression profiles constitutes a powerful tool in order to identify genes and pathways related to the host response to infections. Here, we analyzed the transcriptomic response of human placental chorionic villi explants (HPCVE) challenged with T. cruzi trypomastigotes at low (105) and high (106) concentrations for 2 and 24 hours
 
Overall design Human Placental Chorionic Villi Explants (HPE) were obtained from term placentas (>38 weeks) with an informed consent from heatlhy women in elective cesarean delivery. In a Level II biosafety cabinet, 0,5 cm3 HPE were isolated from the center of the cotyledon, washed with sterile PBS in order to remove excess of blood and later incubated with 105 or 106 parasites/ml (Y Strain Tripomastigotes, the infective form of the parasite) for 2 and 24 hrs.
 
Contributor(s) Castillo C, Carrillo I, Libisch G, Juiz N, Schijman A, Robello C, Kemmerling U
Citation(s) 30143027
Submission date Apr 16, 2018
Last update date Sep 13, 2018
Contact name Ulrike Kemmerling
E-mail(s) ukemmerling@uchile.cl
Phone +5629786261
Organization name Universidad de Chile
Department Faculty of Medicine
Lab Anatomy and developmental biology
Street address Independencia 1027
City Santiago
ZIP/Postal code 8380453
Country Chile
 
Platforms (1)
GPL16699 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version)
Samples (17)
GSM3098157 HPCVE Control 2 hrs, 1
GSM3098158 HPCVE Control 2 hrs, 2
GSM3098159 HPCVE Control 2 hrs, 3
Relations
BioProject PRJNA450333

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113155_RAW.tar 51.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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