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| Status |
Public on Dec 17, 2019 |
| Title |
WDR5 regulates EMT and metastasis in breast cancer by activating TGFB pathway [RNA-seq PDX] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The core subunit of the COMPASS-like complex, WD Repeat Domain 5 (WDR5) has a prominent role in reprogramming and Epithelial-to-Mesenchymal transition (EMT) in different tumor types. Our evidences support a model in which WDR5 is prominent for EMT and metastasis dissemination in breast cancer patient-derived xenografts and cell lines. Moreover, WDR5 silencing abrogates TGFB pathway activation and reverts mesenchymal into epithelial phenotype, by inhibiting transcription of main master regulators of EMT (CDH2, TWIST1, SNAI1, SNAI2 and ZEB1). Our data suggest that WDR5 inhibition may be a successful approach to prevent progression of metastatic BC.
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| Overall design |
Total RNA from human breast cancer metastatic patient-derived xenografts (PDXs) line was processed for multiparallel sequencing. Experiments were carried out in shLuc (control) and WDR5 silenced cells
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| Contributor(s) |
Balestrieri C, Punzi S, Lanfrancone L |
| Citation(s) |
31752957 |
| |
| Submission date |
Apr 17, 2018 |
| Last update date |
Dec 17, 2019 |
| Contact name |
Chiara Balestrieri |
| E-mail(s) |
balestrieri.c@gmail.com
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| Organization name |
IRCCS San Raffaele Scientific Institute
|
| Department |
Center for Omics Sciences
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| Street address |
Via Olgettina 58
|
| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE113289 |
WDR5 regulates EMT and metastasis in breast cancer by activating TGFB pathway |
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| Relations |
| BioProject |
PRJNA450653 |
| SRA |
SRP140715 |
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