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Series GSE113385 Query DataSets for GSE113385
Status Public on Feb 12, 2019
Title Gene expression profiling in mouse fibroblast tumors with overexpression of the homeoprotein SIX1
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Six1 is a developmental transcriptional regulator frequently overexpressed in human tumors. Recent results also show that SIX1 acts as a repressor of cell senescence, an antiproliferative response with key roles in tumor suppression, among other physiological and pathological settings. Here, we set to study the impact of SIX1 gain of function in transformation and tumorigenesis of fibroblasts, in connection with senescence. Using transcriptomic, histological, and functional analyses in murine cells and tumors of fibroblast origin, we show that SIX1 has a strong pro-tumorigenic action in this model, linked to the repression of a senescence-related gene signature and the activation of cellular plasticity, mediated at least in part by direct transcriptional regulation of the stemness factor Sox2. Moreover, functional analyses with human glioma cell lines also show that SIX1 controls SOX2 expression, senescence and self-renewal in this model. Collectively, our results support a general link of SIX1 with senescence and SOX2-mediated cell plasticity in tumors.
 
Overall design mRNA profiles were obtained from SIX1-overexpressing tumors and controls in triplicate by RNA-Seq using Illumina HiSeq.
 
Contributor(s) Palmero I
Citation(s) 30723235
Submission date Apr 19, 2018
Last update date Mar 15, 2022
Contact name Ignacio Palmero
E-mail(s) ipalmero@iib.uam.es
Organization name CSIC
Street address Arturo Duperier, 4.
City Madrid
State/province Madrid
ZIP/Postal code 28029
Country Spain
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM3104681 V-RAS rep1
GSM3104682 V-RAS rep2
GSM3104683 V-RAS rep3
Relations
BioProject PRJNA450993
SRA SRP141174

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113385_RNASEQ_SIX1.txt.gz 551.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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