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| Status |
Public on Apr 20, 2021 |
| Title |
Shh is a determinant of gammadelta T-cell development in the thymus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We compared gammadelta T-cell development and subsets in the thymus of mice in which Shh had been conditionally deleted from thymic epithelial cells using FoxN1-Cre to WT mice, and to transgenic mice in which a constitutively activator (Gli2DN2) or constitutuvely repressor (Gli2DC2) form of Gli2 were expressed under the control of the lck-promotor. We sorted CD27+CD3+TCR-gammadelta+ cells from 4 week old mice and comapred gene expression by RNAsequencing. All mice were on a C57BL/6 background.
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| Overall design |
Wildtype, Gli2A, Gli2R and Shhfl/fl FoxN1 Cre+ (n=2) 4 week old thymi were sorted for CD3+CD27+gdTCR+ cells. RNA extracted from these cells was sequenced to help understand the transcriptional changes governing gd T cell development.
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| Contributor(s) |
Crompton T, Mengrelis K |
| Citation(s) |
31379834 |
| |
| Submission date |
Apr 20, 2018 |
| Last update date |
Jul 14, 2021 |
| Contact name |
Tessa Crompton |
| Organization name |
UCL
|
| Department |
Immunobiology
|
| Street address |
30 Guilford Street
|
| City |
London |
| ZIP/Postal code |
WC1N 1EH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA451195 |
| SRA |
SRP141427 |
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