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| Status |
Public on Jan 18, 2019 |
| Title |
Measuring A-to-I RNA editing signatures of neuronal populations within the Drosophila brain |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
We used an improved INTACT (Isolation of Nuclei Tagged in A specific Cell Type) technique to isolate RNA from purified nuclei from different neuronal populations of the Drosophila brain. Using microfluidic multiplex PCR and sequencing (mmPCR-seq), we determined gene expression and A-to-I RNA editing levels at editing sites across nine distinct neuronal sub-populations and a pan-neuronal control.
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| Overall design |
We crossed UAS-unc84-2XGFP transgenic flies with 10 different GAL4 drivers (Dh44-GAL4, NPF-GAL4, NPFR-GAL4, Tdc2-GAL4, Crz-GALl4, TH-GAL4, Trh-GAL4, Fru-GAL4, OK107-GAL4, and elav-GAL4), immunoprecipitated tagged nuclei and extracted RNA. Three independent replicates of each each cross were performed to isolate RNA subjected to mmPCR-seq for targeted RNA editing analysis.
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| Contributor(s) |
Sapiro AL, Shmueli A, Gilbert LH, Li Q, Shalit T, Yaron O, Pass Y, Li JB, Shohat-Ophir G |
| Citation(s) |
30659150, 33867948 |
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| Submission date |
Apr 25, 2018 |
| Last update date |
May 25, 2021 |
| Contact name |
Galit Shohat-Ophir |
| E-mail(s) |
galit.ophir@biu.ac.il
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| Organization name |
Bar Ilan University
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| Street address |
The Nanotechnology Building 206, Room C-663
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| City |
Ramat Gan |
| ZIP/Postal code |
5290002 |
| Country |
Israel |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE113663 |
A-to-I RNA editing signatures of neuronal populations within the Drosophila brain |
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| Relations |
| BioProject |
PRJNA453552 |
| SRA |
SRP142611 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE113662_RAW.tar |
410.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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