|
| Status |
Public on Jun 01, 2020 |
| Title |
Influence of G. lamblia infection on the transcriptional profile in intestinal and liver tissue [liver] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
G. lamblia is a fecal-oral transmitted human enteropathogenic protozoan with extremely high incidence in endemic areas in Africa and Asia particularly in the infant population. It homes to the proximal small intestine and induced diarrhea and malabsoption. Here we established a novel murine G. lamblia infection model and analyzed the tissue response and downstream microbial and metabolic effects in the host.
|
| |
|
| Overall design |
Neonate mice at the age of 3-4 days were orally infected by gavage with 2x10^5 G. lamblia ATCC 5058 (assemblage B, strain GS, clone H7) trophozoites. The infection was monitored by antigen ELISA and quantitative PCR using weekly fecal samples as well as histology, transcriptional analysis of total gut tissue, isolated intestinal epithelial cells (Lotz et al., JEM, 2006) and total liver tissue as well as metabolic analysis in blood, intestinal content and liver tissue.
|
| |
|
| Contributor(s) |
Hornef MW, Riba AL, Hassani K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 25, 2018 |
| Last update date |
Jun 23, 2023 |
| Contact name |
Mathias W. Hornef |
| E-mail(s) |
mhornef@ukaachen.de
|
| Phone |
+491702210495
|
| Organization name |
RWTH Áachen University
|
| Department |
Medical Microbiology
|
| Street address |
Steppenbergallee 203
|
| City |
Aachen |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL24228 |
Agilent-084107 048306On1M_V3 082895 [PROBE NAME VERSION] |
|
| Samples (16)
|
|
| This SubSeries is part of SuperSeries: |
| GSE113668 |
Influence of G. lamblia infection on the transcriptional profile in intestinal and liver tissue |
|
| Relations |
| BioProject |
PRJNA453571 |
Less...
More...