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| Status |
Public on May 08, 2008 |
| Title |
Candidate prostate cancer genes through comparative expression-profiling of seminal vesicle |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
BACKGROUND: Prostate cancer is the most frequently diagnosed cancer among men in the United States. In contrast, cancer of the seminal vesicle is exceedingly rare, despite that the prostate and seminal vesicle share similar histology, secretory function, androgen dependency, blood supply, and (in part) embryonic origin. We hypothesized that gene-expression differences between prostate and seminal vesicle might inform mechanisms underlying the higher incidence of prostate cancer.
METHODS: Whole-genome DNA microarrays were used to profile gene expression of 11 normal prostate and 7 seminal vesicle specimens (including 6 matched pairs) obtained from radical prostatectomy. Supervised analysis was used to identify genes differentially expressed between normal prostate and seminal vesicle, and this list was then cross-referenced to genes differentially expressed between normal and cancerous prostate. Expression patterns of selected genes were confirmed by immunohistochemistry using a tissue microarray.
We identified 32 genes that displayed a highly statistically-significant expression pattern with highest levels in seminal vesicle, lower levels in normal prostate, and lowest levels in prostate cancer. Among these genes was the known candidate prostate tumor suppressor GSTP1 (involved in xenobiotic detoxification). The expression pattern of GSTP1 and four other genes, ABCG2 (xenobiotic transport), CRABP2 (retinoic acid signaling), GATA3 (lineage-specific transcription) and SLPI (immune response), was confirmed by immunohistochemistry.
CONCLUSIONS: Our findings identify candidate prostate cancer genes whose reduced expression in prostate (compared to seminal vesicle) may be permissive to prostate cancer initiation. Such genes and their pathways may inform mechanisms of prostate carcinogenesis, and suggest new opportunities for prostate cancer prevention.
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.
Disease State: normal prostate vs normal seminal vesicle sample
Individual
Keywords: Logical Set
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| Overall design |
cDNA microarrays from the Stanford Functional Genomics Facility were used for expression profiling of 11 normal prostate and 7 seminal vesicle specimens (6 of which were matched pairs), against a universal RNA reference. Extracted expression ratios were normalized by array then mean centered by gene, and expression differences between normal prostate and seminal vesicle identified using Significance Analysis of Microarrays (SAM).
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| Contributor(s) |
Thompson M |
| Citation(s) |
18500686 |
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| Submission date |
May 07, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Jonathan Pollack |
| E-mail(s) |
pollack1@stanford.edu
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| Phone |
650-736-1987
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| Organization name |
Stanford University
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| Department |
Pathology
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| Lab |
Pollack Lab
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| Street address |
269 Campus Drive, CCSR3245A
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5176 |
| Country |
USA |
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| Platforms (1) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA106485 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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