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| Status |
Public on May 02, 2018 |
| Title |
Cardiac-specific developmental and epigenetic functions of Jarid2 during embryonic development |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
Jarid2 cooperates with PRC2 for H3K27me3 accumulation on a subset of Jarid2 target genes in the developing heart, which contributes to repress differentiation of other lineages such as neural differentiation, and to guide normal myocardial development.
Jarid2 forms a functional complex with PRC2 to increase or maintain H3K27me3 levels on the specific promoters in the developing heart.
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| Overall design |
Sonicated chromatin from 20 pooled E17.5 fixed hearts was immunoprecipitated using H3K27me3 antibody, followed by the reversal of cross-linking and DNA purification. Immuno-enriched DNA targets were amplified by whole genome amplification and fluorescently labeled, which were then hybridized onto the Roche NimbleGen 3X720K RefSeq promoter arrays and scanned with an Axon 4000B.
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| Contributor(s) |
Cho E, Lee Y |
| Citation(s) |
29891551 |
| |
| Submission date |
May 01, 2018 |
| Last update date |
Jun 27, 2018 |
| Contact name |
Youngsook Lee |
| E-mail(s) |
youngsooklee@wisc.edu
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| Organization name |
University of Wisconsin-Madison
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| Department |
CRB
|
| Street address |
1111 Highland Ave
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| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
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| Platforms (1) |
| GPL16605 |
NimbleGen_MM9_3x720K_Refseq_Promoter [090618_MM9_Refseq_Prom_ChIP] |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA454380 |
