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| Status |
Public on Jun 01, 2008 |
| Title |
Expression profiling in transgenic FVB/N embryonic stem cells overexpressing STAT3. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the importance of STAT3 in the establishment of ES cells we have in a first step derived stable pluripotent embryonic stem cells from transgenic FVB mice expressing a conditional tamoxifen dependent STAT3-MER fusion protein. In a second step, STAT3-MER overexpressing cells were used to identify STAT3 pathway-related genes by expression profiling in order to identify new key-players involved in maintenance of pluripotency in ES cells.
Keywords: Comparative expression analysis, STAT3 pathway related genes
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| Overall design |
FVB/N derived embryonic stem cells cultivated in the presence of LIF were compared with FVB/N ES cells overexpressing STAT3-MER and cultivated in the presence of 4-hydroxytamoxifen and absence of LIF
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| Contributor(s) |
Cinelli P, Casanova EA, Uhlig S, Lochmatter P, Matsuda T, Yokota T, Rülicke T, Ledermann B, Bürki K |
| Citation(s) |
18500982 |
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| Submission date |
May 09, 2008 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Paolo Cinelli |
| E-mail(s) |
paolo.cinelli@ltk.uzh.ch
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| Phone |
+41446355461
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| Fax |
+41446356875
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| Organization name |
University of Zurich
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| Department |
Institute of Laboratory Animal Science
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| Lab |
Molecular Genetics
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| Street address |
Winterthurerstrasse 190
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| City |
Zurich |
| ZIP/Postal code |
CH-8057 |
| Country |
Switzerland |
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| Platforms (3) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
| GPL83 |
[MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array |
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| Samples (18)
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| GSM287673 |
FVB/N Embryonic Stem Cells overexpressing STAT3-MER, replicate 1 |
| GSM287674 |
FVB/N Embryonic Stem Cells overexpressing STAT3-MER, replicate |
| GSM287675 |
FVB/N Embryonic Stem Cells overexpressing STAT3-MER, replicate 3 |
| GSM287676 |
Wildtype FVB/N Embryonic Stem Cells, replicate 1 |
| GSM287677 |
Wildtype FVB/N Embryonic Stem Cells, replicate 2 |
| GSM287678 |
Wildtype FVB/N Embryonic Stem Cells, replicate 3 |
| GSM287691 |
FVB/N Embryonic Stem Cells overexpressing STAT3-MER, replicate 1, Chip B |
| GSM287692 |
FVB/N Embryonic Stem Cells overexpressing STAT3-MER, replicate 3, Chip B |
| GSM287693 |
Wildtype FVB/N Embryonic Stem Cells, replicate 1, Chip B |
| GSM287694 |
Wildtype FVB/N Embryonic Stem Cells, replicate 2, Chip B |
| GSM287725 |
Wildtype FVB/N Embryonic Stem Cells, replicate 3, Chip B |
| GSM287726 |
Wildtype FVB/N Embryonic Stem Cells, replicate 3, Chip C |
| GSM287727 |
Wildtype FVB/N Embryonic Stem Cells, replicate 2, Chip C |
| GSM287728 |
Wildtype FVB/N Embryonic Stem Cells, replicate 1, Chip C |
| GSM287729 |
FVB/N Embryonic Stem Cells overexpressing STAT3-MER, replicate 1, Chip C |
| GSM287730 |
FVB/N Embryonic Stem Cells overexpressing STAT3-MER, replicate 2, Chip C |
| GSM287731 |
FVB/N Embryonic Stem Cells overexpressing STAT3-MER, replicate 3, Chip C |
| GSM287756 |
FVB/N Embryonic Stem Cells overexpressing STAT3-MER, replicate 2, Chip B |
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| Relations |
| BioProject |
PRJNA106525 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11398_RAW.tar |
130.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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