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| Status |
Public on May 05, 2018 |
| Title |
Transcriptomic analysis of Wt Mtb H37Rv and WhiB4 Complemented strains on treatment with 0.25 mM Cumene hydroperoxide (CHP) for 2 h at 37°C. |
| Organism |
Mycobacterium tuberculosis H37Rv |
| Experiment type |
Expression profiling by array
|
| Summary |
WhiB4 regulates expression of anti-oxidant genes and survival of Mtb in resposne to oxidaitve stress. Thus, we did microarray upon exposure to 0.25 mM CHP for Wt Mtb and MtbΔwhiB4 strain complemented with whiB4 gene to study the restoration of gene expression to wild type levels.
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| Overall design |
Wt Mtb H37Rv and WhiB4 Complemented strains were grown till early log phase and exposed to 0.25 mM CHP for 2 hours at 37 degree at 150rpm. Three independent experiments were done for each of the strain, respectively.
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| Contributor(s) |
Anand K, Mishra S, Singh A |
| Citation(s) |
30149290 |
| |
| Submission date |
May 04, 2018 |
| Last update date |
Sep 06, 2018 |
| Contact name |
SAURABH MISHRA |
| E-mail(s) |
saurabhsls1@gmail.com
|
| Organization name |
Weill Cornell Medicine
|
| Department |
Microbiology and Immunology
|
| Street address |
1300 York Ave, Box 62, New York
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16972 |
UDSC M. tuberculosis H37Rv Validated Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA461796 |
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