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| Status |
Public on Nov 13, 2019 |
| Title |
Importance of AMPK in metformin suppression of liver glucose production |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Metformin is now the most widely prescribed oral anti-diabetic agent worldwide, taken by over 150 million people annually. Although metformin has been used clinically to treat type 2 diabetes for over 60 years. Its mechanism of action remains only partially understood and controversial. In particular, this includes whether AMPK plays a role in metformin suppression of liver glucose production. To address this issue, we knocked out the AMPK catalytic alpha1 and alpha 2 subunits in the liver of HFD-fed adult homozygous mice. These mice were treated with a physiological relevent metformin dose (50 mg/kg/day) for 3 weeks. Liver samples were collected.
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| Overall design |
6 weeks old male floxed homozygous AMPK alpha1/2 mice were fed an HFD for 4 weeks, AAV8-TBG-E.V. and AAV8-TBG-Cre (1X10^11GC/mouse) were injected via jugular vein, and mice were treated with metformin (50 mg/kg/day) for 3 weeks, liver samples were collected after 16 h fast. There are 14 samples, 6 samples from control group (WT), another 8 samples from treated group (Cre KO).
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| Contributor(s) |
He L |
| Citation(s) |
31693892 |
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| Submission date |
May 09, 2018 |
| Last update date |
Nov 13, 2019 |
| Contact name |
Ling He |
| Organization name |
The Johns Hopkins University School of Medicine
|
| Department |
Department of Pediatrics-Division of Endocrinology
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| Street address |
600 North Wolfe St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA470636 |
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