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| Status |
Public on Mar 09, 2020 |
| Title |
LATS kinase-mediated selective disruption of CTCF genomic binding |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We identified CTCF as a substrate of the LATS kinases. Under cellular stress conditions that activated LATS, CTCF was phosphorylated in a LATS-dependent manner and lost DNA-binding activity. LATS signaling target genes resided in CTCF-mediated insulated neighborhoods and depended on such chromatin organization to sustain their expression. Genome-wide CTCF DNA-binding profiling revealed that metabolic stress reduced CTCF occupancy specifically at a small subset of CTCF-binding sites that encompassed many LATS target genes and were most significantly associated with LATS signaling. Dissociation of CTCF from LATS target genes disrupted corresponding CTCF-mediated chromatin domains and downregulated LATS target gene expression.
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| |
|
| Overall design |
CTCF ChIP-seq for WT and Glucose free medium treatment in MCF7 breast cancer cells
|
| |
|
| Contributor(s) |
Lu J, Luo H |
| Citation(s) |
32128389 |
| |
| Submission date |
May 10, 2018 |
| Last update date |
Mar 09, 2020 |
| Contact name |
Jianrong Lu |
| E-mail(s) |
hcluo2008@hotmail.com
|
| Organization name |
University of Florida
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Jianrong Lu
|
| Street address |
2033 Mowry RD
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (4)
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|
| Relations |
| BioProject |
PRJNA470938 |
| SRA |
SRP145351 |
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