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| Status |
Public on Jun 14, 2018 |
| Title |
Paternal malnutrition programs offspring’s breast cancer risk and tumor metabolism in a mouse model (MBD-seq) |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We found that LP daughters have lower birthweight, alterations in mammary gland morphology and higher rates of mammary cancer. Further, we found that mammary glands and tumors of LP daughters are metabolic rewired, with alterations in the AMPK and amino-acid metabolism pathways. These changes were associated with differential expression of miRNAs 451a, miR-200c and miR-92a. Importantly, some of the same miRNAs and target genes alterations were detected in breast tumors of women from populations with high rates of low birthweight.
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| Overall design |
Male mice were fed AIN93G-based diets containing either 17.7% (control) or 8.9% (LP) energy from protein from 3 to 10 weeks of age. Males on either group were mated to females raised on a control diet. Female offspring control and LP fathers were treated with 7,12-dimethylbenz[a]anthracene (DMBA) to initiate mammary carcinogenesis. Mature sperm from fathers and mammary tissue from female offspring were used for DNA methylation analyses.
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| Contributor(s) |
Santana R, Carney EJ, Clarke J, Cao H, Cruz MI, Benitez C, Jin L, Fu Y, Cheng Z, Wang Y, de Assis S |
| Citation(s) |
30165877 |
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| Submission date |
May 14, 2018 |
| Last update date |
Nov 01, 2018 |
| Contact name |
Lu Jin |
| E-mail(s) |
lj74@georgetown.edu
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| Organization name |
Georgetown University
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| Department |
Oncology
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| Lab |
NRB Room W405B
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| Street address |
3970 Reservoir Rd NW
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| City |
Washington |
| State/province |
D.C. |
| ZIP/Postal code |
20057 |
| Country |
USA |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE114423 |
Paternal malnutrition programs offspring’s breast cancer risk and tumor metabolism in a mouse model |
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| Relations |
| BioProject |
PRJNA471326 |
| SRA |
SRP145631 |
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