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| Status |
Public on Jul 15, 2018 |
| Title |
Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach |
| Organism |
Clostridioides difficile |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Clostridioides difficile is the major pathogen causing diarrhea following antibiotic treatment. It is considered to be a strictly anaerobic bacterium, however, previous studies have shown a certain and strain-dependent oxygen tolerance. In this study, the model strain C. difficile 630Δerm was shifted to micro-aerobiosis and was found to stay growing to the same extent as anaerobically growing cells for the duration tested. However, an extensive change in gene expression was determined by RNA-Seq with 49 effected genes after 15 min and more than 400 after one hour. Effected genes exhibit a wide range of functionality, including the metabolism of amino acids, enzymes for cell wall synthesis, the uptake and metabolism of sugars, synthesis of B vitamins as well as purine synthesis and DNA repair. Our study suggests that tolerance of C. difficile towards O2 is based on a complex and far-reaching adjustment of global gene expression.
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| Overall design |
Comparative examination of gene expression in Clostridioides difficile grown anaerobically and in the presence of 5% oygen.
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| Contributor(s) |
Neumann-Schaal M, Nuss A, Beckstette M, Dersch P, Sievers S |
| Citation missing |
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| |
| Submission date |
May 15, 2018 |
| Last update date |
Jul 17, 2018 |
| Contact name |
Michael Beckstette |
| Organization name |
Helmholtz Centre for Infection Research
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| Department |
Molecular Infection Biology
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| Lab |
Experimental Immunology
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| Street address |
Inhoffenstrase 7
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| City |
Braunschweig |
| State/province |
Lower Saxony |
| ZIP/Postal code |
38124 |
| Country |
Germany |
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| Platforms (1) |
| GPL22304 |
Illumina HiSeq 2000 (Clostridioides difficile) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA471499 |
| SRA |
SRP147455 |
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