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| Status |
Public on Aug 09, 2018 |
| Title |
Quantitative analysis of Id1 Wild Type and Id1 knockout hematopoietic stem cells transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To explore the molecular basis by which Id1 loss promotes HSC quiescence under stress we compared the transcriptome of Id1-/- and Id1+/+ HSCs isolated from primary BMT recipient mice by RNA-seq. We found 179 upregulated and 1476 downregulated genes in Id1-/- HSCs compared to Id1+/+ HSCs using a fold-change cut-off of 2 . Collectively, the IPA analysis shows that Id1-/- HSCs have gene expression profiles consistent with reduced response to stress, reduced cycling and proliferation, and reduced ribosomal biogenesis and protein synthesis, which is consistent with our hypothesis that Id1-/- HSCs have molecular signature of quiescent HSCs compared to Id1+/+ HSCs.
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| Overall design |
RNA was isolated from HSCs FACS purified from mice transplanted with Lin- Id1-/- and Id1+/+ BMCs after 6 weeks for RNA-seq analysis.
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| Contributor(s) |
Singh SK, Singh S, Gadomski S, Sun L, Keller JR |
| Citation(s) |
30082068 |
| |
| Submission date |
May 16, 2018 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Jack Chen |
| Organization name |
NIH
|
| Street address |
SAIC-Frederick Miller Dr
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA471700 |
| SRA |
SRP147556 |
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