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Series GSE114533 Query DataSets for GSE114533
Status Public on Aug 09, 2018
Title Quantitative analysis of Id1 Wild Type and Id1 knockout hematopoietic stem cells transcriptome
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To explore the molecular basis by which Id1 loss promotes HSC quiescence under stress we compared the transcriptome of Id1-/- and Id1+/+ HSCs isolated from primary BMT recipient mice by RNA-seq. We found 179 upregulated and 1476 downregulated genes in Id1-/- HSCs compared to Id1+/+ HSCs using a fold-change cut-off of 2 . Collectively, the IPA analysis shows that Id1-/- HSCs have gene expression profiles consistent with reduced response to stress, reduced cycling and proliferation, and reduced ribosomal biogenesis and protein synthesis, which is consistent with our hypothesis that Id1-/- HSCs have molecular signature of quiescent HSCs compared to Id1+/+ HSCs.
Overall design RNA was isolated from HSCs FACS purified from mice transplanted with Lin- Id1-/- and Id1+/+ BMCs after 6 weeks for RNA-seq analysis.
Contributor(s) Singh SK, Singh S, Gadomski S, Sun L, Keller JR
Citation(s) 30082068
Submission date May 16, 2018
Last update date Feb 11, 2019
Contact name Jack Chen
Organization name NIH
Street address SAIC-Frederick Miller Dr
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (10)
GSM3143633 Id1 KO-Stressed (S)_A
GSM3143634 Id1 KO-Stressed (S)_B
GSM3143635 Id1 KO-Normal (N)_A
BioProject PRJNA471700
SRA SRP147556

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Supplementary file Size Download File type/resource
GSE114533_LimmaVoom_Normalized_data.txt.gz 1.3 Mb (ftp)(http) TXT
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