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| Status |
Public on Dec 18, 2018 |
| Title |
Genome wide analysis of chick limb bud tissue treated with FGF2 for 24hrs (microarray) |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
During limb development, fibroblast growth factors (FGFs) govern proximal-distal outgrowth and patterning. FGFs also synchronize developmental patterning between the proximal-distal and anterior-posterior axes by maintaining sonic hedgehog (SHH) expression in cells of the zone of polarizing activity (ZPA) in the distal posterior mesoderm. SHH, in turn, maintains FGFs in the apical ectodermal ridge (AER) which caps the distal tip of the limb bud. Crosstalk between FGF and SHH signaling is critical for patterned limb development, but the mechanisms underlying this feedback loop are not well characterized.
Implantation of FGF beads in the proximal posterior limb bud can maintain SHH expression in the former ZPA domain (evident 3hrs after application), while prolonged exposure (24hrs) can induce SHH outside of this domain. Although temporally and spatially disparate, comparative analysis of transcriptome data from these different populations enriched molecules required for FGF-mediated SHH regulation.
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| Overall design |
Total RNA was extracted from SHH-expressing chick limb bud tissue following 24hrs of FGF2 treatment and compared to PBS-treated control
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Authors state that they are "unable to locate the CEL files".
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| Contributor(s) |
Watson BA, Feenstra JM, Van Arsdale JM, Rai-Bhatti KS, Kim DH, Coggins AS, Mattison GL, Steinman ES, Pira CU, Gongol BR, Oberg KC |
| Citation(s) |
29914077 |
| |
| Submission date |
May 18, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Kerby Clyde Oberg |
| E-mail(s) |
koberg@llu.edu
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| Organization name |
Loma Linda University
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| Department |
Pathology and Human Anatomy
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| Lab |
Molecular Embryopathy
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| Street address |
24785 Stewart Street
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| City |
Loma Linda |
| State/province |
CA |
| ZIP/Postal code |
92350 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE114663 |
Genome wide analysis of chick limb bud tissue treated with FGF2 for 3 hrs |
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| Relations |
| BioProject |
PRJNA472076 |
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