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Series GSE11474 Query DataSets for GSE11474
Status Public on Jun 01, 2008
Title Rapid encoding of information alters the profile of plasticity related mRNA transcripts in the hippocampal CA1 region.
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary A theoretical framework for the function of the medial temporal lobe system in memory defines differential contributions of the hippocampal subregions with regard to pattern recognition retrieval processes and encoding of new information. To investigate molecular programs of relevance, we designed a spatial learning protocol to engage a pattern separation function to encode new information. After background training, two groups of animals experienced the same new training in a novel environment, however only one group was provided spatial information and demonstrated spatial memory in a retention test. Global transcriptional analysis of the microdissected subregions of the hippocampus exposed a CA3 pattern that was sufficient to clearly segregate spatial learning animals from control. Individual gene and functional group analysis anchored these results to previous work in neural plasticity. From a multitude of expression changes, increases in camk2a, rasgrp1 and nlgn1 were confirmed by in situ hybridization. Furthermore, siRNA inhibition of nlgn1 within the CA3 subregion impaired spatial memory performance, pointing to mechanisms of synaptic remodeling as a basis for rapid encoding of new information in long-term memory.
Keywords: behavioral intervention: spatial learning paradigm
 
Overall design RNA samples from animals subjected to a spatial learning paradigm were compared to controls using Affymetirx RAE230a chips. An N of 6 was used in each of the two experimental conditions.
 
Contributor(s) Haberman RP, Lee HJ, Colantuoni C, Koh MT, Gallagher M
Citation(s) 18650386
Submission date May 16, 2008
Last update date Mar 03, 2017
Contact name Carlo Colantuoni
E-mail(s) ccolantu@jhmi.edu
Phone 4104931439
Organization name Johns Hopkins Univ. School of Medicine
Department Neurology
Street address 733 N Broadway
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platforms (1)
GPL341 [RAE230A] Affymetrix Rat Expression 230A Array
Samples (12)
GSM289072 Control CA1, biological rep1
GSM289073 Control CA1, biological rep2
GSM289074 Control CA1, biological rep3
This SubSeries is part of SuperSeries:
GSE11476 Rapid encoding of new information alters the profile of plasticity related mRNA transcripts in the hippocampal region
Relations
BioProject PRJNA109121

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11474_RAW.tar 26.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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