|
| Status |
Public on May 23, 2018 |
| Title |
Unwavering Pathobiology of Volumetric Muscle Loss Injury |
| Organism |
Sus scrofa domesticus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Volumetric muscle loss (VML) resulting from extremity trauma presents chronic and persistent functional deficits which ultimately manifest disability. Acellular biological scaffolds, or decellularized extracellular matrices (ECMs), embody an ideal treatment platform due to their current clinical use for soft tissue repair, off-the-shelf availability, and zero autogenous donor tissue burden. ECMs have been reported to promote functional skeletal muscle tissue remodeling in small and large animal models of VML injury, and this conclusion was reached in a recent clinical trial that enrolled 13 patients. However, numerous other pre-clinical reports have not observed ECM-mediated skeletal muscle regeneration. The current study was designed to reconcile these discrepancies. The capacity of ECMs to orchestrate functional muscle tissue remodeling was interrogated in a porcine VML injury model using unbiased assessments of muscle tissue regeneration and functional recovery. Here, we show that VML injury incites an overwhelming inflammatory and fibrotic response that leads to expansive fibrous tissue deposition and chronic functional deficits, which ECM repair does not augment.
|
| |
|
| Overall design |
RNA-Seq time couse of muscle volumetric muscle loss injury healing with controls
|
| |
|
| Contributor(s) |
Ricke DO, Aguilar C |
| Citation(s) |
29030619 |
| |
| Submission date |
May 22, 2018 |
| Last update date |
May 24, 2018 |
| Contact name |
Darrell Orlyn Ricke |
| E-mail(s) |
Darrell.Ricke@ll.mit.edu
|
| Phone |
1-781-981-8323
|
| Organization name |
MIT Lincoln Laboratory
|
| Department |
Group 48 Bioengineering Systems & Technology
|
| Lab |
Bioinformatics
|
| Street address |
244 Wood Street
|
| City |
Lexington |
| State/province |
MA |
| ZIP/Postal code |
02421-6426 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24919 |
Illumina HiSeq 2500 (Sus scrofa domesticus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA472640 |
| SRA |
SRP148771 |
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