|
| Status |
Public on Nov 15, 2008 |
| Title |
Gene expression analysis of ctrl_islets versus VhlhKO_islets |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Understanding the nature of the various glucose-derived signals for insulin secretion (both triggering and amplifying) is essential for gaining insight into the functional failure of the beta-cells in diabetes and the development of drugs for correcting this problem. The beta-cells uniquely couple changes in cellular metabolism to electrical activity and thus insulin release. In mice, beta-cell specific deletion of the von Hippel-Lindau (VHL) tumor suppressor protein leads to the activation of a HIF transcription program that includes genes involved in glycolysis, suppression of mitochondrial activity and lactate production. This reprogramming of cellular metabolism results in abnormal insulin secretion properties.
|
| |
|
| Overall design |
Three batches of isolated islets from each genotype where used for RNA isolation and Affymetrix measurements.
|
| |
|
| Contributor(s) |
Zehetner J, Danzer CF, Krek W |
| Citation(s) |
19056893 |
| |
| Submission date |
May 16, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Jens Zehetner |
| E-mail(s) |
jens.zehetner@cell.biol.ethz.ch
|
| Organization name |
ETH Zurich
|
| Department |
Cell Biology
|
| Lab |
HPM
|
| Street address |
Schafmattstrasse 18
|
| City |
Zurich |
| State/province |
Zurich |
| ZIP/Postal code |
8093 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA106409 |
Less...
More...