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Series GSE115030 Query DataSets for GSE115030
Status Public on Jun 11, 2019
Title A subset of skin macrophages modulates surveillance and regeneration of local nerves
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Host-environment interfaces such as the dermis comprise tissue macrophages as the most abundant resident immune cell type. Diverse tasks, i.e. to resist against invading pathogens, to attract bypassing immune cells from penetrating vessels and to aid tissue development and repair require a dynamic postnatal coordination of tissue macrophages specification. Here, we delineated the postnatal development of dermal macrophages and their differentiation into distinct subsets by adapting single cell transcriptomics, fate-mapping and tissue imaging. We thereby identified a small phenotypically and transcriptionally distinct subset of embryo-derived skin macrophages that was maintained and largely excluded from the overall postnatal exchange by monocytes. These macrophages specifically interacted with dermal sensory nerves, surveilled and trimmed the myelin sheets and regulated axon sprouting after mechanical injury. In summary, our data show long-lasting functional specification of macrophages in the dermis that is driven by step-wise adaptation to guiding structures and ensures codevelopment of ontogenetically distinct cells within the same compartment.
Overall design Single Cell Sequencing was performed on CD45+CD11b+CD64+Lin-(lineage B220, CD3, NK1.1, Siglec-F, Ly6G) CX3CR1 (low, mid, high) macrophage subsets from mouse dermis after enzymatic digestion
Contributor(s) Zeis P, Kolter J, Grün D, Henneke P
Citation(s) 31201094
Submission date May 29, 2018
Last update date Sep 10, 2019
Contact name Patrice Zeis
Organization name Max Planck Institute of Immunobiology and Epigenetics
Lab Dominic Grün
Street address Stübeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (16)
GSM3163376 MacP2_1
GSM3163377 MacP2_2
GSM3163378 MacP2_3
This SubSeries is part of SuperSeries:
GSE114526 Genome-wide expression study of macrophage subsets in mouse skin
BioProject PRJNA473536
SRA SRP149193

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Supplementary file Size Download File type/resource
GSE115030_RAW.tar 7.6 Mb (http)(custom) TAR (of TSV)
GSE115030_macrophages_counttable_allsamples.RData.gz 7.1 Mb (ftp)(http) RDATA
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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