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| Status |
Public on Dec 30, 2009 |
| Title |
Identification of flap structure specific endonuclease 1 as a factor involved in LTM formation of aversive learning |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
We proposed that DNA recombination/repair processes play a role in memory formation. Here, we used microarray analysis of rat amygdala genes to identify possible DNA recombination/repair factors involved in memory consolidation of conditioned taste aversion (CTA). Among the genes that showed statistically significant differential expression, we identified fen-1, encoding a flap-structure specific DNA endonuclease. Amygdalar fen-1 mRNA induction was associated to the illness component of CTA, since it could be observed by the pairing of a flavor and gastrointestinal illness, by the illness itself, but not by the presentation of the flavor alone. No CTA related induction of fen-1 expression was observed in the insular cortex. Importantly, functional validation studies demonstrated that amygdalar suppression of fen-1 expression impaired memory consolidation of CTA. Overall, our studies helped identify a new DNA recombination/repair candidate factor involved in memory formation of aversive experiences.
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| Overall design |
Two comparisons were established between the cRNA samples: CTA versus Flavor-only and CTA versus Toxin-only. For each comparison, the microarray experiment was repeated four times using new biological samples, each consisting of a sample pool from 3 animals. Two of the four biological replicates were performed as dye-swaps in order to correct for dye bias effects.
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| Contributor(s) |
Saavedra- Rodríguez L, Ortiz-Zuazaga HG, Andrés L, Ramírez F, Rodríguez K, Vázquez A, Peña de Ortiz S |
| Citation missing |
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| Submission date |
May 20, 2008 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Sandra Pena de Ortiz |
| E-mail(s) |
sandra@hpcf.upr.edu
|
| Phone |
787-764-0000 ext 7957
|
| Fax |
787-764-3875
|
| URL |
http://fgrc.hpcf.upr.edu
|
| Organization name |
University of Puerto Rico
|
| Department |
Department of Biology
|
| Lab |
Functional Genomics Research Center
|
| Street address |
Gandara Ave., JGD Bldg. Rooms 108, 204
|
| City |
San Juan |
| State/province |
PR |
| ZIP/Postal code |
00931 |
| Country |
Puerto Rico |
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| Platforms (1) |
| GPL890 |
Agilent-011868 Rat Oligo Microarray G4130A (Feature Number version) |
|
| Samples (8)
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| GSM289885 |
experimental CTA versus Flavor only swap1 biological sample1 |
| GSM289886 |
experimental CTA versus Toxin swap1 biological sample1 |
| GSM289907 |
experimental CTA versus Flavor only swap2 biological sample2 |
| GSM289908 |
experimental CTA versus Toxin swap2 biological sample2 |
| GSM289909 |
experimental CTA versus Toxin swap1-biological sample3 |
| GSM289910 |
experimental CTA versus Flavor only swap1-biological sample3 |
| GSM289911 |
experimental CTA versus Flavor only swap2 biological sample4 |
| GSM289912 |
experimental CTA versus Toxin swap2-biological sample4 |
|
| Relations |
| BioProject |
PRJNA106353 |
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