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| Status |
Public on Dec 27, 2018 |
| Title |
Systematic evaluation of C. elegans lincRNAs with CRISPR knockout mutants [lncRNA-seq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Background: LincRNAs play critical roles in eukaryotic cells, but systematic analyses of lincRNAs of an animal for phenotypes have been missing. We have generated CRISPR knockout strains for C. elegans lincRNAs and have evaluated their phenotypes
Results: C. elegans lincRNAs demonstrate global features such as shorter length and fewer exons than mRNAs. For the systematic evaluation of C. elegans lincRNAs, CRISPR knockout strains for 155 out of all the 170 C. elegans lincRNAs were produced. Mutants of 23 lincRNAs have shown phenotypes in 6 traits analyzed. We have investigated these phenotypic lincRNAs for their gene expression patterns and their potential functional mechanisms. Some C. elegans lincRNAs play cis roles to modulate the expression of their neighboring genes, and some other lincRNAs play trans roles as ceRNAs against microRNAs. LincRNAs are extensively regulated by transcription factors, and we dissect the pathway that two transcription factors UNC-30 and UNC-55 control the expression of linc-73 together. Furthermore, linc-73 plays a cis role to modulate the expression of its neighboring gene unc-104, and thus regulates the formation of presynapses.
Conclusions: By using CRISPR/cas9 technology, we have generated knockout strains of 155 C. elegans lincRNAs as valuable resources for studies in noncoding RNAs, and we provide biological insights for 23 phenotypic lincRNAs identified from 6 traits examined.
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| Overall design |
C. elegans mRNA profiles of 9 different stages (N2_embryo, N2_L1, N2_L2, N2_L3, N2_L4, N2_Young_Adult, N2_dauer, N2_starved_mixture, him-5) were generated by deep sequencing, using Illumina HiSeq 2500 and BGISEQ-500.
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| Contributor(s) |
Wei S, Li J |
| Citation(s) |
30621757 |
| |
| Submission date |
Jun 04, 2018 |
| Last update date |
Jan 27, 2019 |
| Contact name |
Ge Shan |
| E-mail(s) |
wyc815@mail.ustc.edu.cn
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| Phone |
86-551-63606274
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| Organization name |
University of Science and Technology of China
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| Department |
School of Life Sciences
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| Street address |
443 Huangshan Road
|
| City |
Hefei |
| State/province |
Anhui |
| ZIP/Postal code |
230027 |
| Country |
China |
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| Platforms (2) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
| GPL25145 |
BGISEQ-500 (Caenorhabditis elegans) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE115324 |
Systematic evaluation of C. elegans lincRNAs with CRISPR knockout mutants |
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| Relations |
| BioProject |
PRJNA474535 |
| SRA |
SRP149736 |
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