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| Status |
Public on Jul 30, 2019 |
| Title |
Transcriptome analysis of wt1b:eGFP positive macrophages in the regenerating zebrafish heart |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Little is known about zebrafish macrophage subtypes and their contribution to organ regeneration. Using the transgenic line wt1b:eGFP we identified a subtype of macrophages in the regenerating heart that is transcriptionally different from the rest of macrophages.
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| Overall design |
Hearts from Tg(wt1b:eGFP; mpeg1:mCherry) zebrafish were cryoinjured and at 4 dpi mCherry and GFP/mCherry cells were FAC sorted. RNA was extracted from 4 pools of GFP/mCherry and 4 pools of mCherry single positive cells and RNAseq was performed.
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| Contributor(s) |
Mercader N, García-Redondo A |
| Citation(s) |
31365871 |
| |
| Submission date |
Jun 05, 2018 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Manuel J Gomez |
| E-mail(s) |
mjgomezr@cnic.es
|
| Organization name |
CNIC
|
| Lab |
Bioinformatics Unit
|
| Street address |
Melchor Fernández Almagro, 3
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE118509 |
Gene expression analysis of wt1b-positive cardiac macrophages |
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| Relations |
| BioProject |
PRJNA474730 |
| SRA |
SRP149832 |
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