|
| Status |
Public on Aug 01, 2018 |
| Title |
Exon Level Machine Learning Analyses Elucidate Novel Candidate miRNA Targets in an Avian Model of Fetal Alcohol Spectrum Disorder |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
An RNA-seq dataset obtained from neural fold-stage chicken (Gallus gallus, strain Special Black) embryos that were exposed to a pharmacologically-relevant alcohol concentration (52 mM for 90 min) or isotonic saline. The cranial headfolds were isolated 6 hours following the initial alcohol exposure. Following RNA isolation, cDNA synthesis, and quality assurance (20), paired-end reads (75 bp) were generated on an Illumina Genome Analyzer IIx (University of Wisconsin Biotechnology Center).
|
| |
|
| Overall design |
Paired end runs with 2 replicate ethanol exposed samples (pool of 23 individual neural folds) and 2 saline control samples (pool of 23 individual neural folds).
|
| |
|
| Contributor(s) |
Al-Shaer AE, Smith SM |
| Citation(s) |
30973878 |
| |
| Submission date |
Jun 05, 2018 |
| Last update date |
Apr 12, 2019 |
| Contact name |
Abrar E Al-Shaer |
| E-mail(s) |
abrar@email.unc.edu
|
| Organization name |
University of North Carolina Chapel Hill
|
| Department |
Nutrition
|
| Lab |
Saame Raza Shaikh
|
| Street address |
135 Dauer Dr
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13797 |
Illumina Genome Analyzer IIx (Gallus gallus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA474737 |
| SRA |
SRP149834 |
Less...
More...