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Status |
Public on Oct 30, 2018 |
Title |
Determination of branch point (BP) usage by wild-type and mutant SF3B1 using a minigene library |
Organisms |
Homo sapiens; Mus musculus; synthetic construct |
Experiment type |
Expression profiling by high throughput sequencing Other
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Summary |
We address define differential usage of branchpoint (BP) in by wild-type SF3B1 SF3B1-K700E using a synthetic mini-gene synthetic library with variable 3' spice sites (3'SS). Minigene-specific libraries were prepared and sequenced on the illumina platform to determine differnces in splice-site usage.
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Overall design |
Minigene-based analysis of differential splicing
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Contributor(s) |
Pillai MM, Paul K, Gupta AK |
Citation(s) |
30462273 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R01 HL098608 |
Cyclin E Regulation in Normal and Neoplastic Hematopoiesis |
BloodCenter of Wisconsin |
Alexander C Minella |
R01 HL104070 |
Role of microRNAs in regulation of the marrow microenvironment |
YALE UNIVERSITY |
Manoj M. Pillai |
R01 HL133406 |
Deregulation of SF3B1 function by recurrent HEAT domain mutations |
YALE UNIVERSITY |
Manoj M. Pillai |
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Submission date |
Jun 10, 2018 |
Last update date |
Mar 27, 2019 |
Contact name |
MANOJ PILLAI |
E-mail(s) |
MANOJ.PILLAI@YALE.EDU
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Organization name |
YALE UNIVERSITY
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Lab |
PILLAI LAB
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Street address |
300 GEORGE STREET #786
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City |
NEW HAVEN |
State/province |
CT |
ZIP/Postal code |
06511 |
Country |
USA |
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Platforms (3) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
GPL15228 |
Illumina HiSeq 2000 (synthetic construct) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (7)
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GSM3181838 |
293T_3'SS library_SF3B1-WT_RNA-seq_ALL |
GSM3181839 |
293T_3'SS library_SF3B1-WT_RNA-seq_selected |
GSM3181840 |
293T_3'SS library_SF3B1-K700E_RNA-seq_ALL |
GSM3181841 |
293T_3'SS library_SF3B1-K700E_RNA-seq_selected |
GSM3181842 |
mESC_3'SS library_SF3B1-K700K_RNA-seq_ALL |
GSM3181843 |
mESC_3'SS library_SF3B1-K700E_RNA-seq_ALL |
GSM3181844 |
3'SS plasmid DNA library_seq |
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Relations |
BioProject |
PRJNA475417 |
SRA |
SRP150151 |