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| Status |
Public on Aug 26, 2018 |
| Title |
Whole blood RNA-Sequencing from 12 Rhesus Macaques following Ebola Makona Infection |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The domestic ferret has recently been described as a uniformly lethal model of infection for three species of Ebolavirus known to be pathogenic to humans. Reagents to systematically analyze the ferret host response to infection are lacking; however, the recent publication of a draft ferret genome has opened the potential for transcriptional analysis of ferret models of disease. In this work, we present comparative analysis of longitudinally sampled blood taken from ferrets and non-human primates infected with lethal doses of the Makona strain of Zaire ebolavirus. Strong induction of proinflammatory and prothrombotic signaling programs were present in both ferrets and non-human primates and both transcriptomes were similar to previously published datasets of fatal cases of human Ebola virus infection.
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| Overall design |
12 Rhesus macaques were infected with 1000 PFU of Ebola virus Makona. Blood samples were taken at pre-infection times as well as 3, 5, and 7 days post infection. Whole blood was collected, inactivated in Trizol, and RNA was extracted for use in RNA-Sequencing.
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| Contributor(s) |
Speranza E, Cross RW, Borisevich V, Widen SG, Wood TG, Shim RS, Adams RD, Gerhardt D, Bennet RS, Honko AN, Johnson JC, Hensley LE, Geisbert TW, Connor JH |
| Citation(s) |
30476250 |
| |
| Submission date |
Jun 13, 2018 |
| Last update date |
Dec 17, 2018 |
| Contact name |
Emily Speranza |
| E-mail(s) |
sperane@ccf.org
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| Organization name |
Cleveland Clinic Lerner Research Institute
|
| Department |
Florida Research and Innovation Center
|
| Street address |
9801 SW Discovery Wy
|
| City |
Port Saint Lucie |
| State/province |
FL |
| ZIP/Postal code |
34987 |
| Country |
USA |
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| Platforms (1) |
| GPL21120 |
Illumina NextSeq 500 (Macaca mulatta) |
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| Samples (60)
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| Relations |
| BioProject |
PRJNA476001 |
| SRA |
SRP150467 |
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