|
| Status |
Public on Oct 24, 2018 |
| Title |
SUMO safeguards somatic and pluripotent cell identities by enforcing distinct chromatin states [SUMO-ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Mouse embryonic fibroblasts (MEFs) were generated from Ubc9fl/- and Ubc9+/+ embryos (E13.5). MEFs were treated with tamoxifen for six days to cause CreERT2 activation, and induce Ubc9 floxed allele deletion. We determined the ChIP-seq profiles of SUMO-1 and SUMO-2 using chromatin of wild-type MEFs Ubc9+/+ and of the corresponding Ubc9 KO MEFs which are entirely depleted for sumoylation. The analysis revealed the nearly complete absence of genome-wide binding of SUMO-1 and SUMO-2 in Ubc9-/- MEFs, attesting for antibody specificities.
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| |
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| Overall design |
One replicate per condiition and mark
|
| |
|
| Contributor(s) |
Cossec J, Theurillat I, Dejean A |
| Citation(s) |
30401455 |
| |
| Submission date |
Jun 15, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Claudia Chica |
| Organization name |
Institut Pasteur
|
| Lab |
Biostatistics and Bioinformatics Hub
|
| Street address |
25-28 Rue du Dr Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA476248 |
| SRA |
SRP150597 |
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