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| Status |
Public on Aug 14, 2018 |
| Title |
Inhibition of aberrant DNA re-methylation improves development of cloned embryos (WGBS-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Somatic cell nuclear transfer (SCNT) enables the genome of a differentiated somatic cell to be reprogrammed to totipotency. However, this process is extremely inefficient, and the underlying mechanism of the epigenetic rearrangements following SCNT remains largely unknown. Here, we generated a genome-wide DNA methylome of mouse SCNT preimplantation embryos. Surprisingly, we identified widespread re-methylated regions (rDMRs) in 2- to 4-cell stage cloned embryos, which caused mis-expression of genes and retrotransposons important for zygotic genome activation and embryo development. Knocking-down DNA methyltransferases can specifically rescue the re-methylation defects of SCNT embryos and evidently improve the poor developmental capacity of cloned embryos. In addition, inactivation of DNA methyltransferases combined with overexpression of histone demethylases led to a more significant reduction in DNA methylation as well as exhibited a synergistic enhancement effect on the full-term development of nuclear transfer embryos. Our study therefore reveals that aberrant re-methylation functions as an unavoidable barrier for SCNT embryo development, and that the removal of multiple epigenetic barriers would be a promising approach to achieve the highest cloning efficiency.
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| Overall design |
For SCNT embryos, 1-2 replicates were performed for each stage.
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| Contributor(s) |
Wang C, Gao R, Xiu W, Liu W, Gao Y, Kou X, Zhao Y, Bai D, Zhang Y, Gao S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 21, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Shaorong Gao |
| Organization name |
Tongji University
|
| Department |
School of life science and technology
|
| Lab |
Gaolab
|
| Street address |
1239 Siping Road, Yangpu District
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (23)
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| This SubSeries is part of SuperSeries: |
| GSE108711 |
Inhibition of aberrant DNA re-methylation improves development of cloned embryos |
|
| Relations |
| BioProject |
PRJNA477280 |
| SRA |
SRP151020 |
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