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| Status |
Public on Jun 23, 2018 |
| Title |
Deficiency in SUMOylation, by injection of Gam1 mRNA into one cell embryos, leads to differential expression of genes in Xenopus |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by array
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| Summary |
Background: Adenovirus protein Gam1 triggers the proteolytic destruction of the E1 SUMO-activating enzyme. Microinjection of an empirically determined amount of Gam1 mRNA into one-cell Xenopus embryos can reduce SUMOylation activity to undetectable, but nonlethal levels, enabling an examination of the role of this post-translational modification during early vertebrate development.
Results: We find that SUMOylation-deficient embryos consistently exhibit defects in neural tube and heart development. We have measured differences in gene expression between control and embryos injected with Gam1 mRNA at three developmental stages: early gastrula (immediately following the initiation of zygotic transcription), late gastrula (completion of the formation of the three primary germ layers), and early neurula (appearance of the neural plate). Although changes in gene expression are widespread and can be linked to many biological processes, three pathways, non-canonical Wnt/PCP, snail/twist, and Ets-1, are especially sensitive to the loss of SUMOylation activity and can largely account for the predominant phenotypes of Gam1 embryos. SUMOylation appears to generate different pools of a given transcription factor having different specificities with this post-translational modification involved in the regulation of more complex as opposed to housekeeping processes.
Conclusions: We have identified changes in gene expression that underlie the neural tube and heart phenotypes resulting from depressed SUMOylation activity. Notably, these developmental defects correspond to the two most frequently occurring congenital birth defects in humans, strongly suggesting that perturbation of SUMOylation, either globally or of a specific protein, may frequently be the origin of these pathologies.
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| Overall design |
Three different time points (early gastrula-9 hours post fertilization, late gastrula- 13.5 hours post fertilization, and early neurula- 16.5 hours post fertilization). Twenty embryos from each time point. Three replicate experiments. Samples from control (injected with water) and experimental (injected with Gam1 mRNA).
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| Contributor(s) |
Bertke MM, Cronin L, Zeng E, Huber PW |
| Citation(s) |
31101013 |
| Submission date |
Jun 22, 2018 |
| Last update date |
Jun 18, 2019 |
| Contact name |
Paul Huber |
| E-mail(s) |
phuber@nd.edu
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| Organization name |
University of Notre Dame
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| Street address |
University of Notre Dame
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| City |
Notre Dame |
| State/province |
IN |
| ZIP/Postal code |
46556 |
| Country |
USA |
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| Platforms (1) |
| GPL10756 |
[X_laevis_2] Affymetrix Xenopus laevis Genome 2.0 Array |
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| Samples (18)
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| GSM3211707 |
Embryo at early gastrula, injected with 0.5ng Gam1 mRNA, biological replicate 1 |
| GSM3211708 |
Embryo at early gastrula, injected with 0.5ng Gam1 mRNA, biological replicate 2 |
| GSM3211709 |
Embryo at early gastrula, injected with 0.5ng Gam1 mRNA, biological replicate 3 |
| GSM3211710 |
Embryo at early gastrula, injected with 9.2nL water, biological replicate 1 |
| GSM3211711 |
Embryo at early gastrula, injected with 9.2nL water, biological replicate 2 |
| GSM3211712 |
Embryo at early gastrula, injected with 9.2nL water, biological replicate 3 |
| GSM3211713 |
Embryo at late gastrula, injected with 0.5ng Gam1 mRNA, biological replicate 1 |
| GSM3211714 |
Embryo at late gastrula, injected with 0.5ng Gam1 mRNA, biological replicate 2 |
| GSM3211715 |
Embryo at late gastrula, injected with 0.5ng Gam1 mRNA, biological replicate 3 |
| GSM3211716 |
Embryo at late gastrula, injected with 9.2nL water, biological replicate 1 |
| GSM3211717 |
Embryo at late gastrula, injected with 9.2nL water, biological replicate 2 |
| GSM3211718 |
Embryo at late gastrula, injected with 9.2nL water, biological replicate 3 |
| GSM3211719 |
Embryo at early neurula, injected with 0.5ng Gam1 mRNA, biological replicate 1 |
| GSM3211720 |
Embryo at early neurula, injected with 0.5ng Gam1 mRNA, biological replicate 2 |
| GSM3211721 |
Embryo at early neurula, injected with 0.5ng Gam1 mRNA, biological replicate 3 |
| GSM3211722 |
Embryo at early neurula, injected with 9.2nL water, biological replicate 1 |
| GSM3211723 |
Embryo at early neurula, injected with 9.2nL water, biological replicate 2 |
| GSM3211724 |
Embryo at early neurula, injected with 9.2nL water, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA477522 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE116164_RAW.tar |
64.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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