|
| Status |
Public on Aug 31, 2018 |
| Title |
Generation of esophageal basal progeitor cells from human pluripotent stem cells |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We established an efficient protocol to generate esophageal epithelial progenitors (EPCs) from human pluripotent stem cells (hPSCs). Inhibition of TGFß and BMP signaling is required for the differentiation of hPSCs into EPCs which can be further purified with EPCAM and Integrin ß4. These purified EPCs express human fetal esophageal genes and recapitulate the normal development of the stratified squamous epithelium. We performed global transcriptomic profiling of E12.5 mouse embryonic esophageal epithelia, human fetal esophageal epithelia and human embryonic stem cell RUES2-derived esophageal progenitor cells through RNA sequencing. We found that these cells shared similar expression of NOTCH components including Jag1 and 2, Dll1, 3 and 4, and Notct1, 3, 4. The gene expression profile allows to understand transcriptional program in mouse and human developing esophagus.
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| |
|
| Overall design |
Analysis of global transcriptomes of E12.5 mouse embryonic esophageal epithelia, human fetal esophageal epthelia and human embryonic stem cell RUES2-derived esophageal progenitor cells.
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| |
|
| Contributor(s) |
Zhang Y, Que J |
| Citation(s) |
30244870 |
| BioProject |
PRJNA477382 |
| |
| Submission date |
Jun 26, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Jianwen Que |
| E-mail(s) |
jq2240@cumc.columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Department of Medicine
|
| Street address |
650 W 168TH ST BLACK BLDG 8-810
|
| City |
NEW YORK |
| State/province |
NJ |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (11)
|
| GSM3223523 |
E12.5 wildtype mouse esophageal epithelium sample_1 |
| GSM3223524 |
E12.5 wildtype mouse esophageal epithelium sample_2 |
| GSM3223525 |
E12.5 wildtype mouse esophageal epithelium sample_3 |
| GSM3223526 |
E12.5 wildtype mouse skin |
| GSM3223527 |
RUES2-derived esophageal progenitor cells sample_1 |
| GSM3223528 |
RUES2-derived esophageal progenitor cells sample_2 |
| GSM3223529 |
RUES2-derived esophageal progenitor cells sample_3 |
| GSM3223530 |
18-week human fetal esophageal epithelium |
| GSM3223531 |
14-week human fetal esophageal epithelium |
| GSM3223532 |
17-week human fetal esophageal epithelium_sample_1 |
| GSM3223533 |
17-week human fetal esophageal epithelium_sample_2 |
|
| Relations |
| SRA |
SRP151089 |
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