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Series GSE11633 Query DataSets for GSE11633
Status Public on Jan 04, 2010
Title Rescue of alpha-synuclein toxicity in a yeast model of Parkinson's disease
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Cellular toxicities of alpha-synuclein manifest through multiple pathways, including mitochondrial dysfunction and the inhibition of vesicle trafficking. Several defects can be ameliorated by small molecule suppressors that antagonize toxicity in model systems ranging from yeast to neurons. Connections between these distinct pathologies may be central to Parkinson Disease and to therapeutic strategies. First, yeast cultures with 1 or 2 copies of human alpha-synuclein were profiled during a time series of 0 to 6 hours. Second, to investigate any potential rescue of alpha-synuclein toxicity, one of a series of six compounds: compound 1 ((4-(3-iodophenyl)-3,4-dihydrobenzo[h]quinolin-2(1H)-one); compound 2 (4-(3-bromophenyl)-3,4-dihydrobenzo[h]quinolin-2(1H)-one); compound 3 (4-(5-bromo-2-fluorophenyl)-6,7-dimethyl-3,4-dihydro-2(1H)-quinolinone); compound 4 (4-(3-bromo-4-fluorophenyl)-6,7-dimethyl-3,4-dihydro-2(1H)-quinolinone); compound 5 (4-(4-ethyl)-6,7-dimethyl-3,4-dihydro-2(1H)-quinolinone); compound 6 ((4R)-6-bromo-4-(4-ethylphenyl)-3,4-dihydrobenzo[h]quinolin-2(1H)-one); was introduced and the expression profile assayed at 4 hours.
 
Overall design For the time series, at each time point (0, 2, 4, 6 hours) three yeast samples (2-copy alpha-synuclein; 1-copy alpha-synuclein; empty vector control) were each prepared and hybridized in all three combinations (2-copy vs control; 1-copy vs control; 2-copy vs 1-copy) for that time point.

For the drug treatment, three yeast samples were grown each with 2-copy alpha-synuclein or an empty vector control, with or without one of six compounds: compound 1 ((4-(3-iodophenyl)-3,4-dihydrobenzo[h]quinolin-2(1H)-one); compound 2 (4-(3-bromophenyl)-3,4-dihydrobenzo[h]quinolin-2(1H)-one); compound 3 (4-(5-bromo-2-fluorophenyl)-6,7-dimethyl-3,4-dihydro-2(1H)-quinolinone); compound 4 (4-(3-bromo-4-fluorophenyl)-6,7-dimethyl-3,4-dihydro-2(1H)-quinolinone); compound 5 (4-(4-ethyl)-6,7-dimethyl-3,4-dihydro-2(1H)-quinolinone); compound 6 ((4R)-6-bromo-4-(4-ethylphenyl)-3,4-dihydrobenzo[h]quinolin-2(1H)-one). 2-color hybridization compared (a) 2-copy vs 2-copy+compound, (b) vector vs vector+compound, or (c) 2-copy+compound vs vector+compound.
 
Contributor(s) Su LJ, Yeger-Lotem E, Bell GW
Citation(s) 20038714
Submission date Jun 02, 2008
Last update date Mar 19, 2012
Contact name Linhui Julie Su
Organization name Whitehead Institite for Biomedical Research
Lab Lindquist Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platforms (1)
GPL1210 OperonYeastV1.1
Samples (93)
GSM284867 1-copy alpha-synuclein vs Vector at time = 0 (#1)
GSM284874 2-copy alpha-synuclein vs Vector at time = 0 (#1)
GSM285648 1-copy alpha-synuclein vs 2-copy alpha-synuclein at time = 0 (#1)
Relations
BioProject PRJNA106153

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11633_RAW.tar 59.7 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table
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