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| Status |
Public on Jan 24, 2020 |
| Title |
Anatomical-spatial dissection of single-cell retinal transcriptomics paves the road for new horizons in complement immunology [Mm] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: We present the first comprehensive map of the murine retinal complement expression at a single-cell resolution from ~92,000 retinal cells, spanning 11 cell types.
Methods: Retinal mRNA profiles from 10-week-old wild-type (WT) Black 6 mouse eyes were generated by scRNA sequencing. The data was normalized using Seurat.
Results: Using an optimized data analysis workflow, we found novel patterns of complement expression in the retina
Conclusions: Our landmark atlas provides crucial mechanistic insights in complement immunology
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| Overall design |
Retinal cell mRNA data from 10-week old wild type (WT) Black 6 eyes were generated by scRNA sequencing.
~92,000 retinal cells, spanning 11 cell types (Amacrine Cells, Cone BCs, Cone Photoreceptors, Endothelial Cells, Horizontal Cells, Microglia, Muller Glia, Pericytes, Retinal Ganglion Cells, Rod BCs, Rod Photoreceptors).
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| Contributor(s) |
Agarwal D, Stambolian D, Dana N |
| Citation(s) |
31775049 |
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| Submission date |
Jun 28, 2018 |
| Last update date |
Jan 24, 2020 |
| Contact name |
Dwight Edward Stambolian |
| E-mail(s) |
stamboli@pennmedicine.upenn.edu
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| Phone |
2158980305
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| Organization name |
University of Pennsylvania
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| Department |
Opthalmalogy
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| Lab |
Stellar-Chance 313
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| Street address |
422 Curie Blvd.
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE117959 |
Cellular milieu of the neural retina informs complement immune regulation |
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| Relations |
| BioProject |
PRJNA478452 |
| SRA |
SRP151599 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE116426_RAW.tar |
2.1 Mb |
(http)(custom) |
TAR (of MTX, TSV, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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