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Series GSE116426 Query DataSets for GSE116426
Status Public on Jan 24, 2020
Title Anatomical-spatial dissection of single-cell retinal transcriptomics paves the road for new horizons in complement immunology [Mm]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: We present the first comprehensive map of the murine retinal complement expression at a single-cell resolution from ~92,000 retinal cells, spanning 11 cell types.
Methods: Retinal mRNA profiles from 10-week-old wild-type (WT) Black 6 mouse eyes were generated by scRNA sequencing. The data was normalized using Seurat.
Results: Using an optimized data analysis workflow, we found novel patterns of complement expression in the retina
Conclusions: Our landmark atlas provides crucial mechanistic insights in complement immunology
Overall design Retinal cell mRNA data from 10-week old wild type (WT) Black 6 eyes were generated by scRNA sequencing.
~92,000 retinal cells, spanning 11 cell types (Amacrine Cells, Cone BCs, Cone Photoreceptors, Endothelial Cells, Horizontal Cells, Microglia, Muller Glia, Pericytes, Retinal Ganglion Cells, Rod BCs, Rod Photoreceptors).
Contributor(s) Agarwal D, Stambolian D, Dana N
Citation(s) 31775049
Submission date Jun 28, 2018
Last update date Jan 24, 2020
Contact name Dwight Edward Stambolian
Phone 2158980305
Organization name University of Pennsylvania
Department Opthalmalogy
Lab Stellar-Chance 313
Street address 422 Curie Blvd.
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (1)
GSM3231460 Mm_retina_Input mRNA
This SubSeries is part of SuperSeries:
GSE117959 Cellular milieu of the neural retina informs complement immune regulation
BioProject PRJNA478452
SRA SRP151599

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Supplementary file Size Download File type/resource
GSE116426_RAW.tar 2.1 Mb (http)(custom) TAR (of MTX, TSV, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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