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| Status |
Public on Sep 17, 2020 |
| Title |
Comparison of epithelial cells from various epithelia and hair follicle stem cells [microarray] |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
The potency of an adult stem cell is restricted to certain lineages during embryonic life, in response to a specific microenvironment (the niche) and it is maintained for life. Lineage restriction is considered immutable. We have investigated if adult stem cells isolated from different epithelia could change fate by exposing them to a hairy skin niche. We have demonstrated that clonogenic stem cells restricted to a single epithelial lineage and cultured from various Tp63-expressing tissues e.g, the bladder the oral mucous, the oesophagus or the thymus can acquire new functionality. When exposed to a hair skin microenvironment, these stem cells acquire the capacity to generate hair follicles and sebaceous glands, thus behaving like bona fide hairy skin multipotent stem cells. Genome wide-screen analysis has demonstrated that the stem cells express transcription factors typical of hair follicle differentiation, even though they continue to express some of the genes linked to their origin. This increase in potency demonstrates that lineage restriction is not immutable.
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| Overall design |
Expression profile difference between hair follicle and cells from various epithelia before and after transplantation into skin
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| Contributor(s) |
Claudinot S, Oshima H, Gonneau C, Bonfanti P, Martens G, Littman D, Nicolas M, Rochat A, Barrandon Y, Pradervand S, Hagenbüchle O |
| Citation(s) |
33159086 |
| |
| Submission date |
Jul 06, 2018 |
| Last update date |
Nov 16, 2020 |
| Contact name |
Sandra Calderon |
| Organization name |
University of Lausanne
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| Department |
Center for Integrative Genomics
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| Lab |
Lausanne Genomics Technologies Facility
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| Street address |
Génopode Building
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| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA479981 |
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