|
| Status |
Public on Jul 07, 2018 |
| Title |
Defining stationary phase transcriptomes of Campylobacter jejuni cell morphotypes |
| Organism |
Campylobacter jejuni |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Filamentation, the transition from rod to filamentous cell morphology, has been identified as a response to stressful conditions in many bacterial species. Campylobacter jejuni exhibits filamentation upon entry to stationary phase. This study examines the cell mophotype specific transciptomes (RNAseq) of C. jejuni.
|
| |
|
| Overall design |
Cell morphotypes were rapidly seperated by filtration from stationary phase Campylobacter jejuni cultures before RNA extraction. Ribosomal RNAs were removed and directional RNAseq perfomed (Illumina HiSeq 2500) to examine cell morphotype-specific transcription at stationary phase compared to short spiral forms harvested from exponential phase growth.
|
| |
|
| Contributor(s) |
Ghaffar NM, Connerton PL, Connerton IF |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 06, 2018 |
| Last update date |
Jul 08, 2018 |
| Contact name |
Ian Frank Connerton |
| E-mail(s) |
ian.connerton@nottingham.ac.uk
|
| Organization name |
University of Nottingham
|
| Department |
School of Biosciences
|
| Lab |
Food Sciences
|
| Street address |
College Road, Sutton Bonington
|
| City |
Loughborough |
| State/province |
Leicestershire |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL19974 |
Illumina HiSeq 2500 (Campylobacter jejuni) |
|
| Samples (9)
|
| GSM3259553 |
C. jejuni PT14 spiral exponential phase replicate 1 |
| GSM3259554 |
C. jejuni PT14 spiral exponential phase replicate 2 |
| GSM3259555 |
C. jejuni PT14 spiral exponential phase replicate 3 |
| GSM3259556 |
C. jejuni PT14 spiral stationary phase replicate 1 |
| GSM3259557 |
C. jejuni PT14 spiral stationary phase replicate 2 |
| GSM3259558 |
C. jejuni PT14 spiral stationary phase replicate 3 |
| GSM3259559 |
C. jejuni PT14 filament stationary phase replicate 1 |
| GSM3259560 |
C. jejuni PT14 filament stationary phase replicate 2 |
| GSM3259561 |
C. jejuni PT14 filament stationary phase replicate 3 |
|
| Relations |
| BioProject |
PRJNA480004 |
| SRA |
SRP152524 |
Less...
More...