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| Status |
Public on Jul 05, 2019 |
| Title |
Initial host response to mycobacterial infection is orchestrated through H3K4 methylation-mediated RNA polymerase II binding at key immune function genes [RNA-seq] |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Massive reprogramming of the host alveolar macrophage transcriptome occurs during the initial stages of tuberculosis. In bovine tuberculosis, Mcobacterium bovis can persist and replicate within alveolar macrophages through varied mechanisms to subvert or exploit host immune responses ( REF). To determine how these transcriptional changes are regulated we performed ChIPseq analysis of H3K4 and H3K27 methylation, established histone tail markers associated with permissive and repressive chromatin states, respectively. These analyses were carried out in parallel with RNA polymerase II ChIPseq, RNAseq and small non-coding RNAseq. This meta data file refers to the RNA-seq datasets.
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| Overall design |
Examination of triple methylation of histone mark H3K4 and H3K27 in bovine alveolar macrophages infected and not infected with Mycobacterium bovis and the resulting change in expression of genes and miRNAs associated with the immune response.
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| Contributor(s) |
Hall TJ, Vernimmen D, Browne JA, Gordon SV, MacHugh DE, O’Doherty AM |
| Citation(s) |
32117424 |
| |
| Submission date |
Jul 06, 2018 |
| Last update date |
Mar 09, 2020 |
| Contact name |
Thomas Hall |
| E-mail(s) |
tjhall1688@gmail.com
|
| Phone |
857168930
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| Organization name |
UCD
|
| Department |
Vet sciences
|
| Street address |
2, Taney Grove
|
| City |
Dublin |
| State/province |
Select One |
| ZIP/Postal code |
D14 KW14 |
| Country |
Ireland |
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| Platforms (1) |
| GPL23295 |
Illumina HiSeq 4000 (Bos taurus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE116734 |
Initial host response to mycobacterial infection is orchestrated through H3K4 methylation-mediated RNA polymerase II binding at key immune function genes. |
|
| Relations |
| BioProject |
PRJNA480009 |
| SRA |
SRP152535 |
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