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| Status |
Public on Aug 14, 2019 |
| Title |
Addressing the repertoire of T helper Cells in the context of myocardial infarction |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
T-cells can influence the post-myocardial infarction healing process. The fundamental role of T cells in adaptive immunity relies mainly on a diverse repertoire of T cell receptor (TCR) α and β chains that display unique antigen specificities. Thus, we performed high-throughput sequencing of the TCRβ chains on CD4+ T cells purified by fluorescence-activated cell sorting (FACS) from the heart and med-LNs of MI and sham-operated mice as an unbiased approach to screen for MI-related TCR signatures.
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| Overall design |
Experimental myocardial infarction (EMI) was induced in Balb/c WT mice by means of ligating the left anterior descending coronary artery. As a sham-operation control, animals were subjected to thoracotomy without coronary ligation. CD4+ T-cells were purified (FACS) from the mediastinal lymph nodes (med-LN, heart-draining) of from the infarcted heart at day 7 post-operation. In addition, we assessed the TCRb repertoire of CD4+ T cells purified from the axillary LNs (ax-LNs) of mice receiving subcutaneous immunization with cardiac myosin and heart extract (in adjuvants), as these repertoires were expected to be enriched in heart-specific sequences upon specific immunization. As a control, we also assessed the ax-LN TCRb repertoire in mice that received subcutaneous administration of the adjuvant alone.
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| Contributor(s) |
Rieckmann M, Hofmann U, Ramos G |
| Citation(s) |
31408441 |
| |
| Submission date |
Jul 13, 2018 |
| Last update date |
Sep 13, 2019 |
| Contact name |
Gustavo Campos Ramos |
| E-mail(s) |
ramos_g@ukw.de
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| Phone |
+49 931 20146477
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| Organization name |
Universitätsklinikum Würzburg
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| Department |
DZHI
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| Lab |
Immunocardiology (AG Ramos)
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| Street address |
AM Schwarzenberg, 15
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| City |
Würzburg |
| ZIP/Postal code |
97078 |
| Country |
Germany |
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| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA481009 |
| SRA |
SRP153371 |
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