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| Status |
Public on Jul 14, 2018 |
| Title |
mRNA and lncRNA expression analysis of irradiated and nonirradiated human HaCaT cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This study aims to compare mRNA expression between radiated and non-radiated human keratinocyte HaCaT cells by microarray analysis. Human keratinocyte HaCaT cells were divided into two groups, each group has three repeats. The cells were irradiated with a single dose of 0 or 20Gy of X-ray irradiation. 48 hours post radiation, cells from 0 or 20 Gy groups were collected and subjected to microarray analysis.
mRNA and lncRNA profilings of each group were analyzed by microarray.
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| Overall design |
This study aims to examine mRNA and lncRNA expression spectrum of irradiated and non-irradiated human keratinocyte HaCaT cells. Human keratinocyte HaCaT cells were divided into two groups, each group has three repeats. The cells were irradiated with a single dose of 0 or 20Gy of X-ray irradiation. 48 hours post radiation, the 0 and 20 Gy irradiated cells were collected by Trizol and subjected to microarray analysis.
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| Contributor(s) |
Zhang S, Jia H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 13, 2018 |
| Last update date |
Oct 29, 2018 |
| Contact name |
huimin Jia |
| E-mail(s) |
hmjia@outlook.com
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| Phone |
18896557779
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| Organization name |
Soochow University
|
| Street address |
No.199 Ren'ai Road, Wuzhong District
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| City |
Suzhou |
| ZIP/Postal code |
215123 |
| Country |
China |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (6)
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| GSM3271219 |
Mock irradiated Human HaCaT cells, biological rep1 |
| GSM3271220 |
20 Gy X-ray irradiated Human HaCaT cells, biological rep1 |
| GSM3271221 |
Mock irradiated Human HaCaT cells, biological rep2 |
| GSM3271222 |
20 Gy X-ray irradiated Human HaCaT cells, biological rep2 |
| GSM3271223 |
Mock irradiated Human HaCaT cells, biological rep3 |
| GSM3271224 |
20 Gy X-ray irradiated Human HaCaT cells, biological rep3 |
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| Relations |
| BioProject |
PRJNA481037 |
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