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| Status |
Public on Jan 01, 2019 |
| Title |
Gene expression changes in THP1 cells at day 2 and 4 following shRNA knock-down of RUVBL2 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used an inducible shRNA system and RNA-Seq to examine gene expression changes in acute myeloid leukemia THP1 cells following silencing of RUVBL2. RUVBL2 is a AAA+ ATPase that functions in a number of cellular processes, including chromatin remodeling and transcriptional control, and is critical for survival of acute myeloid leukemia cells and in vivo disease progression.
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| Overall design |
Total cellular RNA was extracted using the RNeasy Plus Mini Kit from THP1 cells transduced with RUVBL2-specific inducible shRNA, following 2 and 4 days exposure to doxycycline or medium controls. In total, 6 pairs of control and doxycycline-treated samples were analysed (3 control and 3 doxycycline-treated for each time-point).
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| Contributor(s) |
Williams O, Armenteros-Monterroso E, de Boer J |
| Citation(s) |
31138842 |
| |
| Submission date |
Jul 13, 2018 |
| Last update date |
Apr 14, 2020 |
| Contact name |
Owen Williams |
| E-mail(s) |
owen.williams@ucl.ac.uk
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| Phone |
+4420790542180
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| Organization name |
UCL GOS ICH
|
| Department |
Cancer section
|
| Street address |
30 Guilford Street
|
| City |
London |
| ZIP/Postal code |
WC1N 1EH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA481053 |
| SRA |
SRP153417 |
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