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| Status |
Public on Mar 26, 2021 |
| Title |
Gene expression profile in E2F1 and E2F2 deficient mice treated with the procarcinogen agent diethylnitrosamine and fed a high fat diet |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate mechanism involved in the development of NAFLD-driven hepatocarcinoma (HCC), we used genome microarray expression profiling. The aim was to identify genes which upregulation could be controlled by E2F1 and/or E2F2 in NAFLD -driven HCC. For that, HCC was induced in wild type (WT), E2f1 knockout mice (E2f1-/-) and E2f2 knockout mice (E2f2-/-) by administration of the procarcinogenic agent diethylnitrosamine (DEN) combined with a feeding of high fat diet (HFD) for 32 weeks.The microarray results were then validated by RT-qPCR. For that, the expression of the selected genes (some overexpressed, some downregulated and others with no change) was measured in the same RNA samples by real time PCR. The results were clustered in pathways with the Reactome Database.
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| Overall design |
The differential gene expression associated to HCC development was measured in livers of WT, E2f1-/- mice, and E2f2-/- mice treated with DEN and fed a HFD. An n of 4 mice was used in each group
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| Contributor(s) |
Mestre D, Gómez B, Aurrekoetxea I, Saénz de Urturi D, Núñez-García M, Rodríguez-García JL, González-Romero F, Fernández-Ares L, Buqué X, Iglesias A, Bernales I, Zubiaga AM, Aspichueta P |
| Citation(s) |
33771899 |
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| Submission date |
Jul 20, 2018 |
| Last update date |
Mar 29, 2021 |
| Contact name |
Patricia Aspichueta |
| E-mail(s) |
patricia.aspichueta@ehu.eus
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| Organization name |
University of the Basque Country UPV/EHU
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| Department |
Physiology
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| Street address |
Barrio Sarriena s/n
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| City |
Leioa |
| ZIP/Postal code |
48940 |
| Country |
Spain |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA482062 |
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