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Series GSE11794 Query DataSets for GSE11794
Status Public on Jul 12, 2008
Title Untreated 32Dcl3 cell lines expressing oncogenic tyrosine kinases or cells treated with small molecule inhibitors
Organism Mus musculus
Experiment type Expression profiling by array
Summary Oncogenic tyrosine kinases, such as BCR-ABL, TEL-ABL, TEL-PDGF-beta-R and FLT3-ITD, play a major role in the development of hematopoietic malignancy. They activate many of the same signal transduction pathways.
To identify the critical target genes required for transformation in hematopoietic cells, we used a comparative gene expression strategy in which selective small molecules were applied to 32Dcl3 cells that had been transformed to factor-independent growth by these respective oncogenic alleles.

Keywords: comparative gene expression analysis
Overall design In our microarray study, we have total 22 samples from four different cell lines expressing BCR-ABL, TEL-ABL, FLT3-ITD or TEL-PDGF-betaR. Each cell line was treated with specific small molecule inhibitors for 4 hours, then RNA was extracted and cRNA was hybridized to Affymetrix U74 oligonucleotide arrays. Untreated cells or cells treated with unrelated inhibitors were used as controls or references. We have three replicates and six references obtained from three independent experiments for cells expressing BCR-ABL or FLT3-ITD. We have one treated and one untreated sample for each cell line expressing TEL-ABL or TEL-PDGF-beta-R.
Contributor(s) Tam WF, L Gu T, Chen J, Lee BH, Bullinger L, Frohling S, Wang A, Monti S, Golub TR, Gilliland DG
Citation(s) 18559972, 21593872
Submission date Jun 16, 2008
Last update date Feb 18, 2018
Contact name Winnie F Tam
Phone 617-355-9079
Fax 617-355-9093
Organization name Brigham and Women's Hospital, Harvard Medical School
Department Hematology
Lab Dr. Gary Gilliland
Street address 1 Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02453
Country USA
Platforms (3)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
GPL82 [MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array
GPL83 [MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array
Samples (66)
GSM299342 C-20376-B/A(untreated1)_U74A
GSM299343 C-20451-B/A(untreated2)_U74A
GSM299344 C-20452-B/A(untreated3)_U74A
BioProject PRJNA105997

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Supplementary file Size Download File type/resource
GSE11794_RAW.tar 178.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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