|
| Status |
Public on Jul 25, 2019 |
| Title |
ChIP-seq of ER in MCF-7 cells post ligand treatment |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Goal: study the impact of estrogen receptor (ER) ligands on ER binding to chromatin in MCF-7 cells
Methods: ER Chromatin Immunoprecipitation and Sequencing (ChIP-seq)
Results: All tested ligands increase binding of ER onto DNA in MCF-7 breast cells. These ligands thus promote an association between ER and DNA, irrespective of their mode of action: selective ER modulator (SERM) 4-OH tamoxifen, and selective ER degraders (SERD) fulvestrant and GDC-0927.
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| |
|
| Overall design |
ChIP-Seq of MCF-7 cells treated with five different estrogen receptor ligands or DMSO for 45 minutes
|
| |
|
| Contributor(s) |
Metcalfe C, Daemen A, Hafner M, Zhou W |
| Citation(s) |
31353221 |
| |
| Submission date |
Jul 31, 2018 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Marc Hafner |
| E-mail(s) |
hafner.marc@gene.com
|
| Organization name |
Genentech
|
| Department |
Oncology Bioinformatics
|
| Street address |
Building 45-1, 1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (7)
|
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| This SubSeries is part of SuperSeries: |
| GSE117943 |
ATAC-seq, ChIP-seq and RNA-seq of breast cancer cell lines post ligand treatment |
|
| Relations |
| BioProject |
PRJNA483772 |
| SRA |
SRP155880 |
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