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Status |
Public on Jul 25, 2019 |
Title |
ChIP-seq of ER in MCF-7 cells post ligand treatment |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Goal: study the impact of estrogen receptor (ER) ligands on ER binding to chromatin in MCF-7 cells Methods: ER Chromatin Immunoprecipitation and Sequencing (ChIP-seq) Results: All tested ligands increase binding of ER onto DNA in MCF-7 breast cells. These ligands thus promote an association between ER and DNA, irrespective of their mode of action: selective ER modulator (SERM) 4-OH tamoxifen, and selective ER degraders (SERD) fulvestrant and GDC-0927.
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Overall design |
ChIP-Seq of MCF-7 cells treated with five different estrogen receptor ligands or DMSO for 45 minutes
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Contributor(s) |
Metcalfe C, Daemen A, Hafner M, Zhou W |
Citation(s) |
31353221 |
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Submission date |
Jul 31, 2018 |
Last update date |
Oct 29, 2019 |
Contact name |
Marc Hafner |
E-mail(s) |
hafner.marc@gene.com
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Organization name |
Genentech
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Department |
Oncology Bioinformatics
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Street address |
Building 45-1, 1 DNA Way
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City |
South San Francisco |
State/province |
CA |
ZIP/Postal code |
94080 |
Country |
USA |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (7)
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This SubSeries is part of SuperSeries: |
GSE117943 |
ATAC-seq, ChIP-seq and RNA-seq of breast cancer cell lines post ligand treatment |
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Relations |
BioProject |
PRJNA483772 |
SRA |
SRP155880 |