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| Status |
Public on Sep 30, 2022 |
| Title |
Chip-seq analysis of meiotic Cohesin complexes (mei-Cohesins) subunits, CTCF, and BORIS/CTCFL in Macaca fascicularis testis |
| Organism |
Macaca fascicularis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The study involved chromatin IP analyzed by high-throughput sequencing analysis using purifird germ cells from M. fascucularis testis
M. fascicularis is a monkey with non-seasonal male germ cells production, and the most popular primate for preclinical animal studies.
The goal of the study was to investigate the epigenome of M. fscicularis testis with respect to mei-Cohesin complexes, CTCF and BORIS
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| Overall design |
The cynomolgus monkeys testis material was collected from six 7-9 years old male monkeys. The testes were placed on ice and germ cells were prepared by passing through two mesh sizes.The cell fraction was fixed according to ChIP-seq protocol and kept frozen
|
| Web link |
http://genome.ucsc.edu/s/Strunnik/cohesins_CTCF_BORIS_K27Ac_macFas5_pub
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| |
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| Contributor(s) |
Boukaba A, Wu Q, Liu J, Li J, Liang J, Strunnikov A |
| Citation(s) |
36179046 |
| Submission date |
Aug 01, 2018 |
| Last update date |
Oct 03, 2022 |
| Contact name |
Alexander V. Strunnikov |
| E-mail(s) |
alexstrunnikov@gmail.com
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| Organization name |
GIBH
|
| Lab |
Molecular Epigenetics
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| Street address |
190 Kai Yuan Avenue
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510530 |
| Country |
China |
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| Platforms (2) |
| GPL23096 |
HiSeq X Ten (Macaca fascicularis) |
| GPL28212 |
Illumina NovaSeq 6000 (Macaca fascicularis) |
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| Samples (29)
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| Relations |
| BioProject |
PRJNA483999 |
| SRA |
SRP156043 |
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