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| Status |
Public on Dec 31, 2018 |
| Title |
Gene expression profile upon TNF-a stimulation, forced expression of NICD, or TNF-a stimulation under forced expression of NICD, in human colon carcinoma-derived LS174T cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A human colon carcinoma-derived cell line LS174T was modified to overexpress NICD, intracellular domain of Notch1, upon doxycycline addition (designated as LS174T-tetON-NICD cells), using the T-rex system (Invitrogen). We have previously shown that these cells can overexpress NICD under the control of CMV promoter (Okamoto R et al, Am J Physiol, 296(1):G23-35, 2009), and the amont of the overexpressed NICD protein reaches to the maximal level in early as 3 hours from doxycycline addition (100ng/ml), which persists for up to 24 hours. In the present experiment, LS174T-tetON-NICD cells were treated with recombinant human TNF-a (50ng/ml) alone, doxycycline alone (100ng/ml), co-treated by both TNF-a and doxycycline, or left untreated (Control) for 24 hours, and subjected for analysis. Experiment was done using a modified sub-line of LS174T cell (LS174T-tetON-NICD cells), in which overexpression of NICD can be induced by a Doxycycline dependent manner.
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| Overall design |
Four-condition experiment, Control vs. TNF-a stimulation, Control vs Doxycycline addition (NICD overexpression) and Control vs TNF-a stimulation+Doxycycline addition (NICD overexpression). Biological replicates: One for each condition, independently grown and harvested. One replicate per array.
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| Contributor(s) |
Okamoto R, Kawamoto A |
| Citation(s) |
30395194 |
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| Submission date |
Aug 02, 2018 |
| Last update date |
Apr 01, 2019 |
| Contact name |
Ryuichi Okamoto |
| E-mail(s) |
rokamoto.gast@tmd.ac.jp
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| Organization name |
Tokyo Medical and Dental University
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| Street address |
Tokyo Medical and Dental University
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| City |
Tokyo |
| State/province |
Please Select |
| ZIP/Postal code |
113-8519 |
| Country |
Japan |
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| Platforms (1) |
| GPL13915 |
3D-Gene Human Oligo chip 25k V2.1 |
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| Samples (3) |
| GSM3317742 |
Control vs TNF-a stimulation |
| GSM3317743 |
Control vs Doxycycline addition (NICD overexpression) |
| GSM3317744 |
Control vs TNF-a stimulation+Doxycycline addition (NICD overexpression) |
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| Relations |
| BioProject |
PRJNA484183 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118048_RAW.tar |
2.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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