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| Status |
Public on Aug 06, 2018 |
| Title |
The Effect of PRMT5 deficiency on thymic iNKT cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Protein arginine methylation is a post-translational modification catalyzed by protein arginine methyltransferase (PRMT). To elucidate the role of PRMT5 in T cells, we generated T-cell specific PRMT5-deficient mice (Prmt5 flox/d Cd4-Cre mice) and found a severe loss of thymic iNKT cells as well as a reduced number in peripheral CD4+ and CD8+ T cells. As iNKT cells were significantly decreased in the stage 1, 2 and 3 of developmental stages, RNA-seq was performed using stage 1 iNKT cells of control and PRMT5-deficient mice. This transcriptome analysis will provide mechanistic insight into how PRMT5 contributes to thymic iNKT cell development.
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| Overall design |
Stage 1 iNKT cells were sorted from thymus of control and Prmt5 flox/D Cd4-Cre mice. Total RNA was extracted and RNA-seq was performed by Ion Proton.
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| Contributor(s) |
Inoue M, Okamoto K, Terashima A, Nitta T, Muro R, Negishi-Koga T, Kitamura T, Nakashima T, Takayanagi H |
| Citation(s) |
30323341 |
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| Submission date |
Aug 05, 2018 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Maia Inoue |
| E-mail(s) |
ino-im@m.u-tokyo.ac.jp
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| Organization name |
The university of Tokyo
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| Street address |
7-3-1, Hongo, Bunkyo-ku,
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| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA484635 |
| SRA |
SRP156398 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118135_Processed_data_file_Inoue.xlsx.gz |
19.6 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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