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| Status |
Public on Jul 23, 2008 |
| Title |
Two PBAP chromatin remodeling complex-specific subunits have distinct, redundant functions during Drosophila development |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Chromatin remodeling complexes control the availability of DNA binding sites to transcriptional regulators. Two distinct forms of the major SWI/SNF-related complex that have different activities in vitro can be distinguished by the presence of specific accessory subunits. In Drosophila, the core Brahma complex associates either with Osa to form the BAP complex, or with Bap170 and Bap180 to form the PBAP complex. Mutations affecting the core subunits have stronger developmental phenotypes than osa mutations; these differences could be due to PBAP complex activity . We have generated mutations in the genes encoding the PBAP-specific subunits Bap170 and Bap180 in order to study their functions in vivo. Bap180 is not essential for viability, but is required in ovarian follicle cells for normal eggshell development. Bap170 is necessary to stabilize the Bap180 protein; however, a mutant form that retains this function is sufficient for survival and fertility. The two subunits act redundantly to allow metamorphosis; using gene expression profiling of double mutants, we have found that the PBAP complex regulates genes involved in tissue remodeling and immune system function. Finally, we have generated mutants that lack Bap170, Bap180 and Osa in the germline to demonstrate that the function of the core Brahma complex in oogenesis does not require any of these accessory subunits.
To study PBAP specific subunits Bap170 and Bap180 role in Drosophila melanogaster development
Keywords: genetic modification, chromatin remodelling, gene regulation, microarray
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| Overall design |
Two males and two females for each genotype (wild type and bap170;bap180 double mutant) were processed at white prepupae stage (0 hour APF)
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| Contributor(s) |
Carrera I, Zavadil J, Treisman JE |
| Citation(s) |
18573871 |
| Submission date |
Jun 18, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jessica E Treisman |
| Organization name |
NYUMC
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| Department |
Cell Biology (Skirball)
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| Lab |
SKI 4 Lab-9
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| Street address |
540 First Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA105921 |
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