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| Status |
Public on Jul 30, 2019 |
| Title |
Comparison of the gene expression expression profile of activated and resting epicardium |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
Zebrafish can regenerate their hearts. As a first response to injury, the epicardium reacts by upregulalion of developmental marker genes. Here we compared in an unbiased manner the expression of the epicardium from uninjured zebrafish hearts and hearts at 3 days postcryoinjury. To label epicardial cells of uninjured hearts we usd pard3:GFP (POon et al 2010) and to label epicardium of the injured heart wt1b:eGFP (Perner et al 2007)
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| Overall design |
GFP positive cells were FAC sorted from uninjured pard3:GFP adult zebrafish hearts and wt1b:eGFP hearts 3 days postinjury (dpi). RNA was extracted from 4 pools of pard3:GFP and 4 pools of wt1b:eGFP cells. Each pool contained GFP positive cells isolated from 15 pard3:GFP hearts or 3 wt1b:eGFP hearts, respectively .
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| Contributor(s) |
Mercader N, González-Rosa JM |
| Citation(s) |
31365871 |
| |
| Submission date |
Aug 13, 2018 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Manuel J Gomez |
| E-mail(s) |
mjgomezr@cnic.es
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| Organization name |
CNIC
|
| Lab |
Bioinformatics Unit
|
| Street address |
Melchor Fernández Almagro, 3
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
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| Platforms (1) |
| GPL14664 |
Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Probe Name version) |
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| Samples (8)
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| GSM3330481 |
R1: resting epicardium (par3:eGFP) sample 1 |
| GSM3330482 |
R2: resting epicardium (par3:eGFP) sample 2 |
| GSM3330483 |
A1: activated epicardium (wt1b:eGFP) sample 1 |
| GSM3330484 |
A2: activated epicardium (wt1b:eGFP) sample 2 |
| GSM3330485 |
R3: resting epicardium (par3:eGFP) sample3 |
| GSM3330486 |
R4: resting epicardium (par3:eGFP) sample 4 |
| GSM3330487 |
A3: activated epicardium (wt1b:eGFP) sample 3 |
| GSM3330488 |
A4: activated epicardium (wt1b:eGFP) sample 4 |
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| This SubSeries is part of SuperSeries: |
| GSE118509 |
Gene expression analysis of wt1b-positive cardiac macrophages |
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| Relations |
| BioProject |
PRJNA485719 |
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