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| Status |
Public on Aug 16, 2018 |
| Title |
A galling insect activates plant reproductive programs during gall development |
| Organism |
Vitis riparia |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Some insects can redirect plant development to form unique organs called galls, which provide these insects with unique, enhanced food and protection from enemies and the elements. Many galls resemble flowers or fruits, suggesting that elements of reproductive development may be involved. We addressed this possibility using RNA sequencing (RNAseq) to quantify the transcriptional responses of wild grapevine (Vitis riparia Michx.) leaves to a galling parasite, phylloxera (Daktulosphaira vitifoliae (Fitch 1855)). If development of reproductive structures is part of gall formation, we expected to find significantly elevated expression of genes involved in flower and/or fruit development in developing galls as opposed to ungalled leaves. We found that reproductive gene ontology classes were significantly enriched in developing galls, and that expression of many putative genes involved in flower formation was significantly increased, particularly in later gall stages. The patterns of gene expression found in galls suggest that phylloxera exploits vascular cambium to provide meristematic tissue and redirects leaf development towards formation of carpels. The phylloxera leaf gall, and perhaps other similar galls, appears to be phenotypically and transcriptionally convergent on the plant carpel.
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| |
|
| Overall design |
Comparison of 4 gall developmental stages (1-4) with 3 ungalled control leaves (small, medium, large)
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| |
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| Contributor(s) |
Schultz JC, Edger PP, Body MJ, Appel HM |
| Citation(s) |
30755671 |
| |
| Submission date |
Aug 15, 2018 |
| Last update date |
Feb 27, 2019 |
| Contact name |
William G. Spollen |
| E-mail(s) |
spollenw@missouri.edu
|
| Phone |
573-884-8151
|
| Organization name |
University of Missouri-Columbia
|
| Department |
Informatics Research Core Facility
|
| Lab |
Scott A. Givan
|
| Street address |
113 Bond Life Sciences Center
|
| City |
Columbia |
| State/province |
MO |
| ZIP/Postal code |
65211 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24498 |
Illumina HiSeq 2000 (Vitis riparia) |
|
| Samples (27)
|
|
| Relations |
| BioProject |
PRJNA486136 |
| SRA |
SRP157983 |
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